山西平陆野葛根愈伤组织诱导与培养条件筛选  

Selection for Callus Induction and Culture Conditions of Pueraria Lobata Come from Pinglu County

作　　者：张静 王刚狮[1] 刘瑞霞[1] 时宝凌[1] 廉梅霞[1] Zhang Jing;Wang Gangshi;Liu Ruixia;Shi Baoling;Lian Meixia(Shanxi Forestry Vocational Technical College,Taiyuan 030009,Shanxi,China)

机构地区：[1]山西林业职业技术学院,山西太原030009

出　　处：《农业技术与装备》2022年第6期29-31,37,共4页Agricultural Technology & Equipment

基　　金：2020年林下重点研发计划专项山西林下植被调查研究。

摘　　要：文章对山西平陆野葛根愈伤组织诱导与培养条件进行了筛选,结果表明:野葛根愈伤组织诱导的最适培养基为MS+1.0 mg/L 6-BA+2.0 mg/L 2,4-D+0.5 mg/L NAA+0.3 mg/L KT;16 h光照+8 h黑暗的光周期最适合于野葛根愈伤组织的生长与总黄酮的合成;在野葛根愈伤组织生长稳定期的末期(培养26 d),细胞中次级代谢产物总黄酮含量最高。The results of the conditions of callus induction and culture,showed that the most suitable medium is MS + 1.0 mg/L 6-BA + 2.0 mg/L 2,4-D + 0.5 mg/L NAA + 0.3 mg/L KT,16 h light and 8 h dark is most suitable for growth and total flavonoidsat the end of the growth stability(culture 26 d).The total flavonoids content of secondary metabolites is the highest.

关 键 词：野葛 愈伤组织诱导 总黄酮 光周期 

分 类 号：S567.239[农业科学—中草药栽培]