基于牵引力显微镜技术的生物力学交叉实验教学项目设计  

Design of multi-disciplinary mechanobiology experimental teaching project based on traction force microscopy

作　　者：杜淑媛 徐越[3] 郭传文 杨春 DU Shuyuan;XU Yue;GUO Chuanwen;YANG Chun(School of Aerospace Engineering,Tsinghua University,Beijing 100084,China;National Demonstration Center for Experimental Mechanics Education,Tsinghua University,Beijing 100084,China;School of Life Sciences,Tsinghua University,Beijing 100084,China)

机构地区：[1]清华大学航天航空学院,北京100084 [2]清华大学国家级力学实验教学示范中心,北京100084 [3]清华大学生命科学学院,北京100084

出　　处：《实验技术与管理》2022年第7期176-180,共5页Experimental Technology and Management

基　　金：北京高校教学改革创新项目(100301001);清华大学本科生教育教学改革经费项目(53411400619)。

摘　　要：细胞与胞外基质相互作用产生的主动作用力叫牵引力,对这个力进行表征需要用细胞牵引力显微镜技术(traction force microscope,TFM),该技术主要是通过测量细胞外弹性基质的变形得到形变场,再通过反演算法重构细胞所产生的牵引力,为许多牵引力失衡所导致的疾病(如癌症、组织纤维化、动脉粥样硬化等)的机理探究、诊断与治疗提供重要的理论支撑和检测手段。该文将这一带有鲜明力学与生物学交叉特色,且具有巨大实际应用前景的先进实验技术引入了生物力学实验教学,并适配了相应的实验内容、实验流程及课程安排。Traction force,an active force generated by interaction between cell and extracellular matrix,which required TFM for characterization.Based on the strain field obtained by measuring substrate deformation data,traction force can be retrieved using inversion algorithms,providing important theoretical support and detection means for mechanism exploration,diagnosis and treatment of many diseases caused by traction force imbalance(such as cancer,tissue fibrosis,atherosclerosis,etc.).This experiment introduces this advanced experimental technology with distinct features of integration of biology and mechanics into the biomechanics experimental teaching.The corresponding experiment content,experiment steps and course arrangement are also adapted.

关 键 词：细胞牵引力显微镜技术 前沿实验 实验教学 

分 类 号：Q-331[生物学] G642.0[文化科学—高等教育学]