不同运动方式对大鼠骨骼肌MSTN和IGF1的影响比较  

Comparison of the effects of different exercise modes on MSTN and IGF1 of rat skeletal muscle

作　　者：刘秀娟[1] 盛蕾 张念云[3] 王子艺 申雪纯 LIU Xiujuan;SHENG Lei;ZHANG Nianyun;WANG Ziyi;SHEN Xuechun(Department of Sports and Health,Nanjing Sports Institute,Nanjing 210014,China;Research Office,Nanjing Sports Institute,Nanjing 210014;Department Science Experiment Center,Nanjing Sports Institute,Nanjing 210014)

机构地区：[1]南京体育学院运动健康学院,南京210014 [2]南京体育学院科研处,南京210014 [3]南京体育学院科学实验中心,南京210014

出　　处：《中国比较医学杂志》2022年第7期41-48,共8页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(31900844);江苏省高校自然科学基金(17KJB180008);南京体育学院重点实验室——运动人体科学实验室课题(SYS202106)。

摘　　要：目的探究不同运动方式对大鼠骨骼肌生长因子MSTN和IGF1的影响,为选择合适的运动方式以改善骨骼肌功能提供理论依据。方法SD大鼠40只,随机分为4组,每组10只,分别为安静对照组、持续游泳运动组、高强度间歇游泳运动组和负重爬梯运动组。各运动组进行为期8周的运动训练后,与安静对照组一起麻醉处死,收集样本。记录各组大鼠的体重、腓肠肌质量,HE染色观察腓肠肌的横截面积变化,ELISA方法检测大鼠血清MSTN、IGF1和胰岛素(insulin)的水平,Western blot方法检测大鼠腓肠肌MSTN、IGF1、p70S6K的表达。结果3个运动组的大鼠体重均显著低于安静对照组(P<0.01),持续游泳运动组腓肠肌质量显著低于安静对照组(P<0.05),其他两组大鼠腓肠肌质量没有显著变化(P>0.05);3个运动组腓肠肌横截面积显著低于安静对照组(P<0.05),爬梯运动组和高强度间歇游泳运动组腓肠肌横截面积显著高于持续游泳运动组(P<0.05)。ELISA结果显示,3个运动组血清IGF1水平均显著低于安静对照组(P<0.01),而MSTN和insulin水平均没有显著变化。爬梯运动组腓肠肌MSTN蛋白表达显著低于安静对照组(P<0.01),而IGF1和P70S6K的蛋白表达显著高于安静对照组(P<0.01),持续游泳运动组腓肠肌p70S6K的蛋白表达也显著高于安静对照组(P<0.05)。结论3种运动方式相比,爬梯运动和高强度间歇游泳运动虽然都上调腓肠肌质量指数,但对骨骼肌MSTN和IGF1的影响不同。爬梯运动下调MSTN的表达,上调IGF1和p70S6K的表达,而高强度间歇游泳运动对骨骼肌MSTN、IGF1和p70S6K的影响不显著。爬梯运动可能通过降低MSTN并增加IGF1和P70S6K的蛋白表达增加腓肠肌横截面积。Objective This study aimed to explore the effects of different exercise modes on skeletal muscle growth factors MSTN and IGF-1 in rats and to provide a theoretical basis for choosing appropriate exercise modes to improve skeletal muscle function. Methods Forty-eight SD rats were randomly divided into four groups, each with 12 rats, namely quiet control, continuous swimming exercise, high-intensity intermittent swimming exercise, and ladder exercise groups. After 8 weeks of exercise training, rats were sacrificed by overdosed anesthesia together with the quiet control group, and samples were collected for testing. The body weight and gastrocnemius muscle weight of each group were recorded. Cross-sectional area changes of the gastrocnemius muscle were observed by HE staining. Serum levels of MSTN, IGF-1 and insulin were measured by ELISA. MSTN, IGF-1 and p70 S6 K expression in the gastrocnemius muscle of rats was detected by Western blot. Results The weights of rats in the three exercise groups were significantly lower than that of the quiet control group(P<0.01). The weight of the gastrocnemius in the continuous swimming exercise group was significantly lower than that of the quiet control group(P<0.05), and the weight of the gastrocnemius in the other two groups did not change significantly(P>0.05). The gastrocnemius cross-sectional area of the three exercise groups was significantly lower than that of the quiet control group(P<0.05). The gastrocnemius cross-sectional area of ladder exercise and high-intensity intermittent exercise groups was significantly higher than that of the continuous swimming group(P<0.05). Serum IGF-1 levels of the three exercise groups were significantly lower than that of the quiet control group(P<0.01), while MSTN and insulin levels did not change significantly. MSTN protein expression in the gastrocnemius of the ladder exercise group was significantly lower than that of the quiet control group(P<0.01), while IGF-1 and p70 s6 k protein expression was significantly higher than in the qu

关 键 词：运动方式 骨骼肌 MSTN IGF1 P70S6K 大鼠 

分 类 号：R-33[医药卫生]