全塑型塑胶跑道面层材料对大鼠静式吸入毒性研究  

Static inhalation toxicity of plastic racetrack surface materials in rats

作　　者：李培宁 刘香梅 黄静怡 李光先 刘颖 孙侠 何树锋 梁俊杰 刘冬虹 LI Peining;LIU Xiangmei;HUANG Jingyi;LI Guangxian;LIU Ying;SUN Xia;HE Shufeng;LIANG Junjie;LIU Donghong(Guangzhou Quality Supervision and Testing Institute,Guangzhou 511447;China.2.South China Agricultural University,Guangzhou 510000)

机构地区：[1]广州质量监督检测研究院,广州511447 [2]华南农业大学,广州510000

出　　处：《中国比较医学杂志》2022年第7期49-57,共9页Chinese Journal of Comparative Medicine

基　　金：广东省自然科学基金面上项目(2021A1515012494);广东省市场监督管理局科技项目(2020CZ03,2021CZ04)。

摘　　要：目的研究全塑型塑胶跑道面层材料对大鼠静式吸入毒性。方法将检疫合格的雌、雄性SD大鼠随机分为4组,分别为30 d正常对照组、30 d染毒组、90 d正常对照组和90 d染毒组,每组16只,雌雄各半,每天吸入1 h,分别染毒30 d和90 d。实验结束后,对各组大鼠进行剖检,检测凝血、血常规、血生化指标,计算脏器系数,并对各脏器进行组织病理学检查。结果与同期正常对照组比较,30 d染毒组雄鼠凝血酶时间(TT)缩短(P<0.05),90 d染毒组雌鼠活化部分凝血活酶时间(APTT)延长(P<0.01)。30 d染毒组雌鼠血清Ca、P降低(P<0.05,P<0.01),雄鼠血清肌酐(Cr)、尿素氮(BUN)升高;90 d染毒组雌鼠血清BUN、P升高(P<0.05,P<0.01)及Na、Cl降低(P<0.01),雄鼠血清BUN、总蛋白(TP)升高(P<0.05)。30、90 d染毒组雄鼠肾上腺系数均增大(P<0.01)。30 d染毒组大鼠肝汇管区轻度肝细胞脂肪变性3例,肺细小支气管上皮脱落3例,肾囊肿1例,睾丸个别曲细精管萎缩1例。90 d染毒组大鼠肝汇管区轻度肝细胞脂肪变性6例,肺细小支气管上皮脱落2例,肾上腺束状带细胞灶性肥大1例,睾丸个别曲细精管萎缩2例,肾个别肾小球毛细血管扩张2例、肾囊肿1例、透明管型4例、矿化1例、肾盂积水1例。结论在本实验条件下,30 d吸入暴露全塑型塑胶跑道面层材料对SD大鼠肝、肺、睾丸、肾影响较轻,90 d吸入暴露对大鼠肾损伤加重。Objective To study the static inhalation toxicity of plastic racetrack surface materials in rats. Methods SD rats that passed quarantine inspection were randomly divided into four groups, namely 30 day normal control, 30 day exposure group, 90 day normal control, and 90 day exposure groups with 16 males and females in each group. Rats were exposed to gases by inhalation for 30 or 90 days. At the end of inhalation exposure, blood coagulation, blood routine test, blood biochemistry, organ coefficient, and organ pathology were assessed. Results Compared with normal control group of the same sex, thrombin time of male rats was shortened in the 30 day exposure group(P<0.05) and activated partial thrombin time of female rats was prolonged in the 90 day exposure group(P<0.01). Serum Ca and P were decreased in female rats(P<0.05, P<0.01), and serum creatinine and urea nitrogen(BUN) in rats were increased in the 30 day exposure group. In the 90 day exposure group, serum BUN and P were increased(P<0.05, P<0.01), Na and Cl were decreased of female rats(P<0.01), whereas serum BUN and total protein were increased in male rats(P<0.05). The adrenal coefficient of male rats was increased in 30 and 90 day exposure groups(P<0.01). In the 30 day exposure group, there were three cases of mild steatosis of hepatocytes in the hepatic portal area, three cases of exfoliation of the bronchoalveolar epithelium, one case of renal cyst, and one case of individual convoluted tubule atrophy of testis. In the 90 day exposure group, there were six cases of mild steatosis of hepatocytes in the hepatic portal area, two cases of exfoliation of the bronchoalveolar epithelium, one case of cellular focal hypertrophy of adrenal zona fasciculata, two cases of individual convoluted tubule atrophy of the testis, two cases of individual glomerular telangiectasia of the kidney, one case of renal cyst, four cases of hyaline cast of the kidney, one case of mineralization, and one case of hydronephrosis. Conclusions The effects on the liver, lung, testis, and

关 键 词：全塑型塑胶跑道面层材料 吸入毒性 大鼠 血液学 脏器毒性 

分 类 号：R-33[医药卫生]