番茄苗期节间长度相关性状差异表达基因鉴定与分析  

Identification and Analysis of Differentially Expressed Genes Related to Internode Length in Tomato Seedlings

作　　者：谷雨 赵亚然 姜晶[1] GU Yu;ZHAO Ya-ran;JIANG Jing(College of Horticulture/Key Laboratory of Facility Horticulture of Ministry of Education,Shenyang Agricultural University,Shenyang 110161,China)

机构地区：[1]沈阳农业大学园艺学院/设施园艺省部共建教育部重点实验室,沈阳110161

出　　处：《沈阳农业大学学报》2022年第3期265-277,共13页Journal of Shenyang Agricultural University

基　　金：国家重点研发计划项目(2019YFD1000300)。

摘　　要：株型是影响作物产量的重要农艺性状,而株高是构成作物株型重要的特征之一,目前在水稻、玉米等大田作物上有较多研究,但在园艺作物尤其是蔬菜作物上关于株型调控的研究较少,更缺乏苗期株型性状的分析。而苗期的适宜株高将有利于茄果类蔬菜定植后缩短缓苗时间,增强抗逆性。选用番茄短节间JZ18和长节间JZ34两个高代自交系,利用外源赤霉素(GA3)、多效唑(赤霉素抑制剂,PAC)、油菜素内酯(BR)和生长素(IAA)分析处理,结果表明GA在调控节间伸长差异中发挥重要作用。构建GA处理的两种番茄品系上胚轴茎组织转录组文库进行测序分析,筛选出表达差异显著的基因SlSAUR71和SlXTH23。利用病毒诱导的基因沉默VIGS技术,结果显示在短节间JZ18中沉默基因SlSAUR71的转化植株表现出节间伸长,而在长节间JZ34上沉默基因SlXTH23后植株表现出节间变短的表型。以上结果表明SlSAUR71和SlXTH23为控制番茄节间长度的关键基因,其中SlSAUR71对番茄节间长度起负向调控作用,而SlXTH23起正向调控作用。研究结果将为进一步深入研究番茄苗期株型特征及宜机化栽培的品种选育提供一定的理论基础。Plant architecture is an important agronomic trait that affects crop yield, and plant height is one of the important characteristics of crop architecture. At present, there are many studies on field crops such as rice and corn. However, there are few studies on plant shape regulation in vegetable crops, especially the analysis of plant architecture traits at seedling stage. The appropriate plant height will be beneficial to shorten the time of slowing seedlings and enhance the stress resistance after the solanaceous vegetables are planted at the seedling stage. In this study, two high-generation inbred lines of tomato short internode JZ18and long internode JZ34 were selected. The treatment of exogenous gibberellin(GA3), paclobutrazol(gibberellin inhibitor, PAC),brassinolide(BR) and auxin(IAA) showed that GA plays an important role in regulating internode elongation. The epicotyl stem tissue transcriptome library of two GA-treated tomato lines was constructed for sequencing analysis, and the genes SlSAUR71 and SlXTH23with significant differences in expression were obtained. Using virus-induced gene silencing(VIGS) technology, we found that the transformed plants that silenced the gene SlSAUR71 in the short internode JZ18 showed internode elongation, while the plants that silenced the gene SlXTH23 in the long internode JZ34 showed internode shortening phenotype. The above results indicated that SlSAUR71 and SlXTH23 were the key genes controlling the length of tomato internodes, among which SlSAUR71 played a negative role in regulating the length of tomato internodes, while SlXTH23 played a positive role in regulating it. The research results will provide a certain theoretical basis for further research on the characteristics of tomato seedlings and the selection of varieties suitable for mechanized cultivation.

关 键 词：赤霉素 番茄 株高 上胚轴长度 下胚轴长度 RNA-SEQ VIGS 

分 类 号：S641.2[农业科学—蔬菜学]