全身动态18 F-FDG PET/CT Patlak显像评价大鼠胶质瘤放疗增敏早期疗效  

Whole-body dynamic 18 F-FDG PET/CT Patlak imaging to evaluate the radiosensitization early efficacy in rat glioma

作　　者：蔡可 张晴晴 余文静 薛杨央[1] 汪会[1] 徐慧琴[1] Cai Ke;Zhang Qingqing;Yu Wenjing;Xue Yangyang;Wang Hui;Xu Huiqin(Dept of Nuclear Medicine,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院核医学科,合肥230022

出　　处：《安徽医科大学学报》2022年第7期1088-1093,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81971643)。

摘　　要：目的探讨全身动态^(18)F-脱氧葡萄糖正电子发射计算机断层显像(^(18)F-FDG PET/CT)Patlak显像评价大鼠C6胶质瘤放疗增敏早期疗效的价值。方法建立24只大鼠C6胶质瘤模型,随机分为对照组、齐墩果酸(OA)组、放疗组、OA+放疗组,每组6只。分别在治疗前及治疗后48 h行75 min全身动态^(18)F-FDG PET/CT Patlak显像,将荷瘤鼠的左心室时间-活度曲线(TAC)作为输入函数用于Patlak分析获得Ki图像,选取动态PET图像最后一帧用于静态分析,观察治疗前后各组最大标准化摄取值(SUV_(max))、Ki值、肿瘤体积变化并记录大鼠存活时间。显像完成后,取出肿瘤组织行肿瘤增殖指数Ki-67及葡萄糖转运蛋白-1(GLUT-1)免疫组化检查。结果治疗前,4组SUV_(max)及Ki值差异无统计学意义(F=0.909、0.858,P>0.05)。治疗后48 h,对照组、OA组的SUV_(max)、Ki值较治疗前升高(t=-10.697、-17.516,P<0.01;t=-19.321、-9.887,P<0.01),放疗组、OA+放疗组的SUV_(max)、Ki值较治疗前下降(t=5.629、6.916,P<0.01;t=7.241、10.490,P<0.01),治疗后48 h放疗组、OA+放疗组SUV_(max)差异无统计学意义(t=1.233,P=0.246),而OA+放疗组的Ki值较放疗组降低,差异有统计学意义(t=3.051,P<0.05)。免疫组化结果显示,Ki-67及GLUT-1在OA+放疗组表达低于其他3组(F=63.887、40.720,P<0.05),此外,Ki-67及GLUT-1与Ki值均呈正相关(r=0.931、0.936,P<0.01)。Kaplan-Meier分析结果显示OA+放疗组荷瘤鼠生存时间较其他3组延长,差异有统计学意义(χ^(2)=19.817,P<0.01)。结论OA对大鼠C6胶质瘤有放射增敏作用,全身动态^(18)F-FDG PET/CT Patlak显像可以评估大鼠C6胶质瘤放疗增敏早期疗效,比PET/CT静态显像更灵敏。Objective To investigate the value of whole-body dynamic ^(18)F-flurodeoxygluse positron emission tomography with computed tomography(^(18)F-FDG PET/CT)Patlak imaging for evaluating the radiosensitization early efficacy in C6 rat glioma.Methods Twenty-four C6 rat glioma models were established and randomly divided into control group,Oleanolic acid(OA)group,radiotherapy group and OA+radiotherapy group,with 6 rats in each group.75 min whole-body dynamic ^(18)F-FDG PET/CT Patlak imaging was performed before and 48 h after treatment.The left ventricular time-activity curve(TAC)of tumor-bearing rats was used as input function for Patlak analysis to obtain Ki images.The last frame of the dynamic PET scan was selected for static analysis,and the changes of maximum standardized uptake value(SUV_(max)),Ki values and tumor volume were observed,and the survival time of tumor-bearing rats was recorded.Then,the tumor samples were dissected for Ki-67 and glucose transporter-1(GLUT-1)immunohistochemical staining.Results Before treatment,there were no significant differences in SUV_(max) and Ki values among the 4 groups(F=0.909,0.858,P>0.05).48 h after treatment,SUV_(max) and Ki values of control group and OA group increased significantly(t=-10.697,-17.516,P<0.01;t=-19.321,-9.887,P<0.01),SUV_(max) and Ki values of radiotherapy group and OA+radiotherapy group decreased(t=5.629,6.916,P<0.01;t=7.241,10.490,P<0.01),there was no significant difference in SUV_(max) between radiotherapy group and OA+radiotherapy group 48 h after treatment(t=1.233,P=0.246),while the Ki values in OA+radiotherapy group was significantly lower than that in radiotherapy group,the difference was statistically significant(t=3.051,P<0.05).The immunohistochemistry staining results suggested that the expression of Ki-67 and GLUT-1 in OA+radiotherapy group was lower than that in the other 3 groups(F=63.887,40.720,P<0.05).In addition,Ki values showed positive correlations with the expression of Ki-67 and GLUT-1(r=0.931,0.936,P<0.01).Kaplan-Meier analysis showed th

关 键 词：全身动态PET/CT Patlak 神经胶质瘤 齐墩果酸 Ki-67 GLUT-1 

分 类 号：R730.5[医药卫生—肿瘤]