药食两用小蓟中细胞毒活性成分解析  

Analysis of Cytotoxic Active Ingredients from Edible and Medicin Cirsium setosum

作　　者：王泽玉 张晓霞 吴勇 王欣 李金杰[1] 戴雪玲 尚小雅[1] Wang Zeyu;Zhang Xiaoxia;Wu Yong;Wang Xin;Li Jinjie;Dai Xueling;Shang Xiaoya(Beijing Key Laboratory of Bioactive Substances and Functional Foods,Beijing Union University,Beijing 100191)

机构地区：[1]北京联合大学生物活性物质与功能食品北京市重点实验室,北京100191

出　　处：《中国食品学报》2022年第7期226-237,共12页Journal of Chinese Institute Of Food Science and Technology

基　　金：北京市教委科技计划重点项目(KZ201811417049)。

摘　　要：本课题组前期研究发现,小蓟乙醇提取石油醚萃取部位具有较好的抑制多种人肿瘤细胞毒活性的作用,现对其中强活性亚组分的化学成分进行研究,挖掘发挥细胞毒活性的功能因子。在体外抑制细胞毒活性指导下,应用多种色谱技术进行分离纯化,采用波谱学方法进行结构鉴定,并结合文献数据对照确定化合物结构。从石油醚的1个活性组分中共分离鉴定了5个甾体化合物,分别为:3β-羟基豆甾-5-烯-7-酮、豆甾-22-烯-3β,5α,6β-三醇、6β-羟基-豆甾-4-烯-3-酮、6β-羟基-豆甾-4,22-二烯-3-酮和6β-羟基-芸苔甾-4-烯-3-酮。5个化合物均为首次从蓟属植物中发现;除化合物α-豆甾-22-烯-3β,5α,6β-三醇外,其余4个化合物对不同人肿瘤细胞均具有一定的抑制活性。The previous research of our group found that the petroleum ether-soluble portion of the ethanol extract of Cirsium setosum had a good inhibitory effects on cytotoxic activity of a variety of human tumor cells in vitro.Now it is necessary to study the chemical composition of unresearched strong active subfractions,and to explore the functional factors that exert cytotoxic activities.Under the guidance of inhibiting cytotoxic activity in vitro,various chromatographic techniques were used for separation and purification,and spectroscopy methods were used for structural elucidation,and the structures were determined by comparison with known literature data.Five steroids were isolated from an active subfraction of the petroleum ether of ethanolic extract of Cirsium setosum,and determined as 3β-hydroxystigmast-5-en-7-one,stigmast-22-ene-3β,5α,6β-triol,6β-hydroxy-stigmasta-4-en-3-one,6β-hydroxy-stigmasta-4,22-dien-3-one and 6β-hydroxycampest-4-en-3-one.To the best of our knowledge,this is the first report showing the presence of all the compounds in the genus Cirsium.In the in vitro assays,except for the stigmast-22-ene-3β,5α,6β-triol,the other four compounds all showed selective cytotoxicities.

关 键 词：小蓟 细胞毒活性 分离纯化 结构鉴定 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]