全反式维甲酸与胃癌预后相关性及其机制研究  

Correlation between ATRA and prognosis of gastric cancer and its related mechanism

作　　者：冯振游 胡孔旺[1] 丁慧明 黄志国 Feng Zhenyou;Hu Kongwang;Ding Huiming;Huang Zhiguo(Dept of General Surgery,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院普外科,合肥230022

出　　处：《安徽医科大学学报》2022年第8期1216-1221,1228,共7页Acta Universitatis Medicinalis Anhui

基　　金：安徽省科技厅重点研究与开发项目(编号:202004j07020036);吴阶平医基金会临床科研专项资助基金(编号:320.6750.19092-19)。

摘　　要：目的探讨全反式维甲酸(ATRA)治疗对胃癌术后患者生存期是否产生影响,分析ATRA是否通过调控上皮间质转化(EMT)对胃癌细胞的增殖、迁移、侵袭和凋亡产生影响。方法选取胃癌术后患者共198例,分为对照组和ATRA组,比较两组间患者生存期的差异。体外培养MGC-803胃癌细胞,分为对照组和ATRA组。CCK-8实验检测其增殖能力,细胞划痕实验检测其迁移能力,Transwel实验检测其侵袭能力,流式细胞术检测凋亡情况。Western blot、RT-qPCR和免疫荧光检测EMT相关基因(E-cadherin、N-cadherin和Vimentin)的表达情况。结果ATRA可以延长胃癌术后患者的生存时间。经ATRA处理后,胃癌细胞增殖、迁移、侵袭能力下降,同时会促进胃癌细胞的凋亡。Western blot、RT-qPCR和免疫荧光结果显示ATRA会下调胃癌细胞N-cadherin和Vimentin的表达水平,上调E-cadherin表达水平。结论ATRA可以延长胃癌术后患者的生存时间,促进胃癌细胞凋亡,通过调控EMT抑制胃癌细胞增殖,迁移,侵袭。Objective To investigate whether all-trans-retinoic acid(ATRA)treatment affects the survival ofgastric cancer patients after surgery,and to analyze whether ATRA affects the proliferation,migration,invasion and apoptosis of gastric cancer cells by regulating epithelial-mesenchymal transition(EMT).Methods A total of 198 patients with gastric cancer after surgery were selected and divided into control group and ATRA group,and the difference in survival time between the two groups was compared.MGC-803 gastric cancer cells were cultured in vitro and divided into control group and ATRA group.The proliferation ability was detected by CCK-8 experiment,the migration ability was detected by cell scratch experiment,the invasion ability was detected by Transwell experiment,and the apoptosis was detected by flow cytometry.The expressions of EMT-related genes(E-cadherin,N-cadherin and Vimentin)were detected by Western blot,RT-qPCR and immunofluorescence.Results ATRA could prolong the survival time of patients after gastric cancer surgery.After ATRA treatment,the proliferation,migration and invasion abilities of gastric cancer cells decreased,and the apoptosis of gastric cancer cells was promoted at the same time.Western blot,RT-qPCR and immunofluorescence results showed that ATRA down-regulated the expression levels of N-cadherin and Vimentin and up-regulated the expression level of E-cadherin in gastric cancer cells.Conclusion ATRA can prolong the survival time of gastric cancer patients after surgery,promote the apoptosis of gastric cancer cells,and inhibit the proliferation,migration and invasion of gastric cancer cells by regulating EMT.

关 键 词：全反式维甲酸 胃肿瘤 预后相关性 上皮间质转化 

分 类 号：R735.2[医药卫生—肿瘤]