宫颈癌患者HPV基因型分布及HPV52编码基因突变情况分析  

Analysis of HPV genotype distribution and HPV52 gene mutation in patients with cervical cancer

作　　者：王利琼 杨雅嵋 江毅敏[1] 袁芳桃[1] 张科荣[2] WANG Li-qiong;YANG Ya-mei;JIANG Yi-min;YUAN Fang-tao;ZHANG Ke-rong(Health Management Center,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637000,China;Department of Obstetrics and Gynecology,Affiliated Hospital of North Sichuan Medical College)

机构地区：[1]川北医学院附属医院健康管理中心,四川南充637000 [2]川北医学院附属医院妇产科

出　　处：《中国病原生物学杂志》2022年第7期810-813,共4页Journal of Pathogen Biology

摘　　要：目的研究本地区宫颈癌患者HPV感染基因型分布特征和HPV52型E6、E7区基因突变情况,为防治宫颈癌提供依据。方法收集本院就诊169例宫颈癌患者样本并进行检测分型。采用PCR热扩增,检测HPV52型E6、E7区基因突变情况。结果169例宫颈癌患者按年龄划分≤30岁、30~40岁、40~50岁、50~60岁、60~70岁和70岁以上分别为3、23、59、58、21和5例。从样本中共计分离出217株HPV病毒,HPV16型105株、HPV18型35株、HPV31型7株、HPV33型9株、HPV35型1株、HPV39型4株、HPV45型1株、HPV51型4株、HPV52型17株、HPV56型3株、HPV58型23株、HPV59型3株、HPV66型3株、HPV68型1株和HPV82型1株。134例患者是单一感染;29例患者双重HPV感染,5例患者三种HPV类型病毒同时感染,1例患者四种HPV类型病毒同时感染。HPV52型E6蛋白编码基因突变情况:单一突变9株,双重突变1株,三重突变1株,四重突变3株。共有14个碱基发生替代,分别为:C114T、C140G、A204G、A232G、G233T、A264T、T272G、G350T、G356A、A379G、T415C、T417A、G425C、G502C和A524G。HPV52型E7蛋白编码基因突变情况:单一突变3株,双重突变3株,三重突变3株,四重突变3株,六重突变1株。共有16个碱基发生替代,具体情况分别为:A577 C、G592 T、A599 G、C611 G、G655 A、G657 A、C662 T、A 690 T、C714 T、T739 A、G742 A、C747 T、C751 T、G 775 A、T 789 A和A 801 G。结论HPV16、18、52、58型是主要基因型,40~60岁是HPV感染高发人群。多数患者携带的HPV52型E6、E7区域的基因存在基因突变,应加强对HPV感染和变异情况监测。Objective To study the distribution characteristics of HPV infection genotypes and HPV52 E6 and E7 gene mutations in cervical cancer patients in this region,so as to provide basis for the prevention and treatment of cervical cancer.Methods The samples of 169 patients with cervical cancer in our hospital were collected and tested.The gene mutations in E6 and E7 regions of HPV52 were detected by PCR thermal amplification.Results According to age≤30 years old,30-40 years old,40-50 years old,50-60 years old,60-70 years old and over 70 years old,169 patients with cervical cancer were divided into 3 cases,23 cases,59 cases,58 cases,21 cases and 5 cases.A total of 217 strains of HPV virus were isolated from the samples,including 105 strains of HPV16,35 strains of HPV18,7 strains of HPV31,9 strains of HPV33,1 strain of HPV35,4 strains of HPV39,1 strain of HPV45,4 strains of HPV51,17 strains of HPV52,3 strains of HPV56,23 strains of HPV58,3 strains of HPV59,3 strains of HPV66,1 strain of HPV68 and 1 strain of HPV82.134 patients were single infection;29 patients had double HPV infection,5 patients were infected with three types of HPV viruses at the same time,and 1 patient was infected with four types of HPV viruses at the same time.Mutation of E6 protein coding gene of HPV52:9 single mutations,1 double mutation,1 triple mutation and 3 quadruple mutations.A total of 14 bases were replaced,including C114 T,C140 G,A204 G,A232 G,G233 T,A264 T,T272 G,G350 T,G356 A,A379 G,T415 C,T417 A,G425 C,G502 C and A524 G.Mutation of E7 protein coding gene of HPV52:3 single mutations,3 double mutations,3 triple mutations,3 quadruple mutations and 1 sixfold mutation.A total of 16 bases were replaced,including A577 C,G 592 T,A599 G,C 611 G,G655 A,G657 A,C662 T,A 690 T,C714 T,T739 A,G742 A,C747 T,C751 T,G775 A,T 789 A and A 801 G.Conclusion HPV16,18,52 and 58 were the main genotypes,and people aged 40 to 60 were the people with high incidence of HPV infection.Most patients had gene mutations in E6 and E7 regions of hpv52,so we should streng

关 键 词：宫颈癌 HPV 基因分型 

分 类 号：R737.33[医药卫生—肿瘤]