Expression Characteristics and Putative Functions of KIF3A/KIF3B During Spermiogenesis of Phascolosoma esculenta  

作　　者：DU Chen MU Danli GAO Xinming LUO Shengyu WANG Jianping JIN Shan ZHU Junquan 

机构地区：[1]Key Laboratory of Applied Marine Biotechnology by the Ministry of Education,Ningbo University,Ningbo 315211,China [2]Ningbo Academy of Oceanology and Fisheries,Ningbo 315012,China

出　　处：《Journal of Ocean University of China》2022年第4期998-1016,共19页中国海洋大学学报（英文版）

基　　金：supported by the Ningbo Science and Technology Plan Projects (Nos. 2019B10016, 2016C 10004);the Major Science and Technology Projects in Zhejiang Province (No. 2011C12013);the Natural Science Foundation of Zhejiang Province (No. LY18C190007);the National Natural Science Foundation of China (No. 312 72642);the Collaborative Innovation Center for Marine High-efficiency and Healthy Aquaculture of Zhejiang Province, and K.C.Wong Magna Fund in Ningbo University

摘　　要：The microtubule(MT)-associated proteins KIF3A and KIF3B are ubiquitously expressed in a wide range of taxa.This study investigated the functions of these proteins in spermiogenesis,which involves various MT-dependent processes,in Phasco-losoma esculenta.We cloned the complete cDNA of Pe-KIF3A/3B.Structural predictions showed that Pe-KIF3A/3B are composed of a highly conserved motor domain,a coiled-coil domain,and a tail domain.Real-time quantitative PCR showed that Pe-kif3a/3b are expressed in all tissues evaluated,with the highest levels in sperm masses.Fluorescence in situ hybridization and immunofluo-rescence were employed to analyze the dynamic expression patterns of KIF3A/3B during spermiogenesis.Pe-KIF3A/3B consistently co-localized with MTs at all stages of spermiogenesis,indicating their potential functions in cargo trafficking.Pe-KIF3A/3B co-localized with mitochondria,suggesting that they may mediate mitochondrial movement.In late-stage spermatids and mature sperm,co-localization was detected in the midpiece,suggesting that Pe-KIF3A/3B could facilitate midpiece formation.The co-localization of Pe-KIF3A and Pe-KIF3B at all stages of spermiogenesis suggests their function as the heterodimeric KIF3AB.Basing on the ob-served temporal and spatial expression patterns of Pe-KIF3A/3B,MTs,and mitochondria in our study,we suggest that heterodimer KIF3AB has a potential role in acrosome biogenesis,sperm head remodeling,enflagellation,and mitochondrial migration during spermiogenesis in P.esculenta.In addition,our study on the morphological characteristics of spermatogenic cells provided fundamental data on the reproductive biology of P.esculenta.

关 键 词：Phascolosoma esculenta SPERMIOGENESIS kinesin 3A/3B HETERODIMERIZATION 

分 类 号：Q954.434[生物学—动物学]