核桃根际土壤一株农用抗生素产生菌的筛选和鉴定  

Screening and identification of an agricultural antibiotic producingstrain from walnut rhizosphere soil

作　　者：卓平清 王瀚[1,2] 赵淑玲 王弋博[3] 叶文斌 田凤鸣 ZHUO Pingqing;WANG Han;ZHAO Shuling;WANG Yibo;YE Wenbin;TIAN Fengming(College of Life Science,Longnan Teacher College,Longnan Gansu 742500;Center for Research and Development of Longnan Characteristic Agro-Bioresource,Longnan Gansu 742500;College of Bioengineering and Biotechnology,Tianshui Normal University,Tianshui Gansu 741001)

机构地区：[1]陇南师范高等专科学校农林技术学院,甘肃陇南742500 [2]陇南特色农业生物资源研究开发中心,甘肃陇南742500 [3]天水师范学院生物工程与技术学院,甘肃天水741000

出　　处：《宁夏师范学院学报》2022年第7期44-51,共8页Journal of Ningxia Normal University

基　　金：甘肃省高等学校科研创新基金资助(2020A-272);国家自然科学基金资助(31960268).

摘　　要：为筛选生防用放线菌,应用平板涂布法、划线分离法、琼脂块法和改良牛津杯法,从成县多个地区核桃根际土壤中分离和筛选出一株高抑菌活性的菌株KS7.结果表明菌株KS7对供试菌株大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌均具有较强的抑菌作用,其琼脂块对三株供试菌株的平均抑菌圈直径分别为31.25 mm、29.72 mm和35.10 mm;其无菌发酵液对三株供试菌株的平均抑菌圈直径分别为17.00 mm、19.17 mm和15.43 mm.根据该菌株的形态学观察、生理生化特性及16S rRNA序列分析,将菌株KS7鉴定为链霉菌属.In order to screen actinomycetes that can produce agricultural antibiotics,a strain KS7 with high antibacterial activity was isolated and screened from walnut rhizosphere soil in many areas of Chengxian county by spread plate method,plat streaking method,agar block method and improvement oxford cup method.The results showed that the strain KS7 had strong antibacterial effect on Escherichia coli,Staphylococcus aureus and Bacillus subtilis.The average antibacterial circle diameters of its agar block on the three strains were 31.25mm,29.72mm and 35.10mm,and the average inhibition zone diameter of the sterile fermentation broth against the three tested strains were 17.00 mm,19.17 mm and 15.43 mm,respectively.According to the morphological observation,physiological and biochemical characteristics and 16S rRNA sequence analysis,the strain KS7 was identified as Streptomyces.

关 键 词：链霉菌 农用抗生素 分离鉴定 抑菌活性 

分 类 号：S432.4[农业科学—植物病理学]