外周血淋巴细胞染色体G显带制备方法的研究进展  

Advances in the Preparation of Chromosome G Banding inPeripheral Blood Lymphocytes

作　　者：景淑贤 陈宝荣 王清涛[3] 于洪远[1] JING Shuxian;CHEN Baorong;WANG Qingtao;YU Hongyuan(Beijing Aerospace General Hospital,Beijing 100076,China;Beijing Jinyu Medical Laboratory Co.,Ltd,Beijing 100015,China;Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China)

机构地区：[1]北京航天总医院,北京100076 [2]北京金域医学检验实验室有限公司,北京100015 [3]首都医科大学附属北京朝阳医院,北京100020

出　　处：《标记免疫分析与临床》2022年第7期1244-1247,共4页Labeled Immunoassays and Clinical Medicine

基　　金：北京市临床重点专科(检验科)卓越项目。

摘　　要：外周血淋巴细胞染色体G显带制备方法在临床应用广泛,作为遗传学诊断中最为基础也是最行之有效的一种检测手段。制备过程繁琐,接种、收获、制片、显带等操作可能都对其最终结果产生一定影响。但现在并没有统一的制备标准,并且进行该操作的实验室环境以及操作人员手法和相关的步骤也有很大的差异,这就导致染色体检查质量有很大区别。本文针对于国内外报道的染色体G显带制备方法进行综述,从而帮助提升染色体制备的核型质量。The preparation of chromosome G banding in peripheral blood lymphocytes has been widely used in clinical practice,and is the most basic and effective classical detection method in clinical genetic diagnosis.The whole preparation process including inoculation,harvest,preparation and banding is complicated,which can affect the final effect of chromosome specimen.However,there is no unified standard for karyotype preparation at_(p)resent.Laboratory conditions and operation procedures are different,resulting in great differences in the effect of chromosome specimen preparation.This paper reviews the improved techniques of chromosome specimen preparation reported at home and abroad in order to improve the quality of chromosome preparation and karyotype analysis.

关 键 词：外周血淋巴细胞 染色体制备 G显带 

分 类 号：R-331[医药卫生]