miR-379和人类驱动蛋白家族成员4A在三阴性乳腺癌中的表达及其关系  

Expression and relationship of miR-379 and human kinesin like family member 4A in triple-negative breast cancer

作　　者：陈丽娟 高学忠[1] 曹茵 袁惠玲 陈桂林[1] Chen Lijuan;Gao Xuezhong;Cao Yin;Yuan Huiling;Chen Guilin(Department of Breast,Affiliated Dongguan Hospital,Southern Medical University(Dongguan People′s Hospital),Dongguan 523000,China)

机构地区：[1]南方医科大学附属东莞医院(东莞市人民医院)乳腺科,东莞523000

出　　处：《中国医师杂志》2022年第7期1028-1031,1036,共5页Journal of Chinese Physician

基　　金：东莞市社会科技发展(重点)项目(2018507150011656)。

摘　　要：目的分析miR-379和人类驱动蛋白家族成员4A(KIF4A)在三阴性乳腺癌(TNBC)中的表达及其关系。方法回顾性选取2016年1月至2019年11月在东莞市人民医院普济分院乳腺科确诊的52例TNBC患者作为研究组,另选取同期确诊的70例非三阴性乳腺癌(NTNBC)患者作为对照组,检测两组患者组织中miR-379、KIF4A的mRNA表达水平,绘制受试者工作特征(ROC)曲线,并计算miR-379、KIF4A预测TNBC的曲线下面积(AUC),分析miR-379、KIF4A表达水平与临床病理参数的关系,并分析miR-379表达水平与KIF4A表达水平的相关性。结果研究组中KIF4A mRNA的表达水平高于对照组[(6.93±0.43)vs(3.75±0.25),P<0.05],miR-379的表达水平低于对照组[(0.54±0.17)vs(0.87±0.32),P<0.05];miR-379联合KIF4A检测对TNBC的诊断价值最大:AUC为0.823(95%CI:0.730~0.917),特异度为0.785,灵敏度为0.950;单因素分析结果显示,不同临床分期、不同肿瘤长径、淋巴结转移与否的TNBC患者miR-379表达比较,差异有统计学意义(均P<0.05);不同临床分期、肿瘤长径的TNBC患者KIF4A mRNA表达比较,差异有统计学意义(均P<0.05);Pearson相关性分析结果显示,TNBC组织中miR-379和KIF4A的表达呈负相关(r=-0.349,P<0.05)。结论TNBC患者组织中miR-379表达下调,KIF4A表达上调,且与不良临床病理特征有关,对患者病情的评估有一定的指导意义。Objective To analyze the expression and relationship of miR-379 and human kinesin family member 4A(KIF4A)in triple-negative breast cancer(TNBC).Methods A total of 52 patients diagnosed with TNBC in breast department at Puji Branch of Dongguan People′s Hospital between January 2016 and November 2019 were retrospectively selected as the study group.Meanwhile,70 patients with non-triple-negative breast cancer(NTNBC)diagnosed in the same period were selected as the control group.The expression levels of miR-379 and KIF4A in both groups were detected.The receiver operating characteristic(ROC)curve was drawn,and the area under the curve(AUC)of miR-379 and KIF4A predicting TNBC was calculated;The relationship between the expression levels of miR-379 and KIF4A and clinicopathological parameters,and the correlation between the expression level of miR-379 and KIF4A were analyzed.Results The expression level of KIF4A in study group was higher than that in control group[(6.93±0.43)vs(3.75±0.25),P<0.05],and the expression level of miR-379 in study group was lower than that in control group[(0.54±0.17)vs(0.87±0.32),P<0.05].MiR-379 combined with KIF4A expression had the greatest diagnostic value for TNBC:AUC 0.823(95%CI:0.730-0.917),specificity 0.785,sensitivity 0.950;Univariate analysis showed that there were significant difference in the expression of miR-379 in TNBC patients with different clinical stages,tumor diameter,and lymph node metastasis(all P<0.05);There were significant difference in the expression of KIF4A in TNBC patients with different clinical stages and tumor diameter(all P<0.05).Pearson correlation analysis showed that miR-379 expression was negatively correlated with KIF4A expression in TNBC(r=-0.349,P<0.05).Conclusions The expression of miR-379 is down-regulated,while the expression of KIF4A is up-regulated in patients with TNBC.The two are related to poor clinicopathological characteristics,which indicates that they can be used for condition evaluation of the patients.

关 键 词：三阴性乳腺癌 miR-379 驱动蛋白家族成员4A 

分 类 号：R737.9[医药卫生—肿瘤]