利于高地芽孢杆菌YC-9生长和芽孢形成的碳源筛选  

Screening of Carbon Sources for Growth and Spore Formation of Bacillus altitudinis YC-9

作　　者：马聪聪 罗泽华 蔡斌 刘好宝 王云山[3] 马锐 顾金刚[2] MA Congcong;LUO Zehua;CAI Bin;LIU Haobao;WANG Yunshan;MA Rui;GU Jingang(Haikou Cigar Research Institute,Hainan Provincial Branch of China National Tobacco Corporation,Haikou 571100,China;Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]中国烟草总公司海南省公司海口雪茄研究所,海口570105 [2]中国农业科学院农业资源与农业区划研究所,北京100081 [3]中国科学院过程工程研究所,北京100190

出　　处：《中国农业科技导报》2022年第7期77-85,共9页Journal of Agricultural Science and Technology

基　　金：海南省烟草公司项目(201746000024502)。

摘　　要：高地芽孢杆菌(Bacillus altitudinis)是烟叶发酵的优势菌之一。通过优化碳源提高高地芽孢杆菌菌株YC-9的生长量和芽孢产量:首先利用Biolog GenⅢ板测定菌株YC-9对71种碳源的代谢特征,然后采用全自动生长曲线仪分析不同碳源对芽孢生物量、生长周期及产孢率的影响,并通过摇瓶发酵确定最佳的产孢条件。结果表明,菌株YC-9可以较好地利用17种碳源,其中4种碳源可以同时促进其生长和产芽孢;在摇瓶水平上使菌株YC-9芽孢产量较多的碳源为L-苹果酸和α-D-葡萄糖,使其芽孢率最高的碳源为葡萄糖+肌苷,培养24 h芽孢总量为3.90×10^(7)cfu·mL^(-1),芽孢率高达60.47%,比以葡萄糖为单一碳源时的芽孢率高约2倍。优化碳源可以显著提高高地芽孢杆菌菌株YC-9的生长、产芽孢率和产芽孢量。Bacillus altitudinisis one of the most predominent bacteria during tobacco fermentation.This study aimed to impove the growth and spore-producing capability of Bacillus altitudinis YC-9 by optimizing the carbon sources.Firstly,the metabolic characteristics of strain YC-9 to 71 kinds of carbon sources were determined by Biolog GenⅢplate,then the effects of different carbon sources on the cell amount,growth cycle and spore yield were studied by using an automatic growth curves analyzer,and the optimal conditions for spore production were determined in shaking flasks.The results showed that strain YC-9 efficiently utilized 17 carbon sources,and 4 of them were favorable for both cell growth and spore production;L-malic acid andα-D-glucose were the better carbon sources for spore production in shake flasks,while glucose and inosine was the highest carbon source for the spore rate.The total amount of spores was 3.90×10^(7)cfu·mL^(-1),and the spore rate was as 60.47%,which was about 2 times higher than that of glucose alone.The cell growth,spore yield,and spore-producing rate of Bacillus altitudinis YC-9 were significantly improved by using optimized carbon sources.

关 键 词：高地芽孢杆菌 BIOLOG 生长曲线 产芽孢率 

分 类 号：S182[农业科学—农业基础科学]