噪声暴露人群的耳钙粘蛋白基因(CDH23)多态性、miR-96、miR-183基因变异性与听力损伤易感相关性  

Correlation of Ear Cadherin Gene(CDH23) Polymorphism,miR-96,miR-183 gene Variability and Susceptibility to Hearing Loss in Noise-exposed People

作　　者：郑雪丽 王成进 陈文渊 李萍 何晓菲 徐学海[3] ZHENG Xueli;WANG Chengjin;CHEN Wenyuan;LI Ping;HE Xiaofei;XU Xuehai(Department of Otorhinolaryngology Head and Neck surgery,Zhangye People's Hospital Affiliated to Hexi University;School of Clinical Medicine,Hexi University;Department of Otolaryngology Head and neck surgery,940 Hospital of PLA Joint Service Support Force)

机构地区：[1]河西学院附属张掖人民医院耳鼻咽喉头颈外科 [2]河西学院临床医学院 [3]中国人民解放军联勤保障部队第940医院耳鼻咽喉头颈外科

出　　处：《中华耳科学杂志》2022年第4期589-594,共6页Chinese Journal of Otology

基　　金：甘肃省自然科学基金项目(20JR10RG303);甘肃省高等学校创新项目(2022A-126)。

摘　　要：目的 探索噪声暴露人群的耳钙粘蛋白基因(CDH23)多态性、miR-96、miR-183基因变异性与听力损伤易感相关性。方法 回顾性分析的259例噪声暴露者均在2019年2月至2020年4月期间收集,根据纯音听力测试、听神经动作电位(CAP)、畸变产物耳声发射(DPOAE)测定结果分为两组,即听力损伤组89例,对照组170例,均进行CDH23、miR-96、miR-183基因检测,分析基因突变检测结果。结果 经二元Logistic回归分析,接触重金属、工龄>5年、高温接触、miR-96基因突变、CDH23基因突变、miR-183基因突变是导致听力受损的独立因子。经相关性分析,听力受损与重金属接触、粉尘接触、高温接触、工龄、CDH23基因、miR-96基因、miR-183基因呈正相关性。同时第七外显子(CDH23-Exon7)的末位CDH23-rs3802711位点基因型和等位基因时,存在统计差异(P<0.05)。结论噪声暴露人群听力损伤与miR-96基因、miR-183基因、CDH23基因多态性变异存在相关性。Objective To determine correlations between ear cadherin gene(CDH23) polymorphism,miR-96 and miR-183 gene variability and susceptibility to hearing damage in noise-exposed people.Methods Data from 259 noiseexposed people collected from February 2019 to April 2020 were retrospectively reviewed,and subjects divided into a hearing impairment group(n=89) and a control group(n=170) based on their pure tone audiometry,auditory nerve compound action potentials(CAPs),and distortion product otoacoustic emissions(DPOAE).All subjects received tests of the CDH23,miR-96 and miR-183 genes for detection of gene mutations.Results Binary logistic regression analysis indicated exposure to heavy metals,working history > 5 years,exposure to high temperature,and mutations of the miR-96,CDH23 or miR-183 genes as independent risk factors for hearing loss.Hearing loss was positively correlated with exposure to heavy metal,dust or high temperature,length of working history,and the CDH23,miR-96 and miR-183genes.Comparison of the last genotype and alleles of the seventh exon yielded statistical differences(P<0.05).Conclusion There is a correlation between hearing loss and polymorphism of the miR-96,miR-183 and CDH23 genes in the noise-exposed population.

关 键 词：噪声 耳钙粘蛋白基因 miR-96 miR-183 基因变异 听力损伤 

分 类 号：R764[医药卫生—耳鼻咽喉科]