2020-2021年市售马立克病疫苗的效价测定及质量评估  

Virus Titer Determination and Quality Evaluation of the Commercial Marek’s Disease Vaccines Sold in 2020-2021

作　　者：郑鹿平 滕蔓 刘金玲[1,2] 罗琴 楚钰淑 王伟东 张文凯 罗俊 ZHENG Luping;TENG Man;LIU Jinling;LUO Qin;CHU Yushu;WANG Weidong;ZHANG Wenkai;LUO Jun(Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences/Ministry of Agriculture and Rural Affairs of China&Henan Provincial Key Laboratory of Animal Immunology,Zhengzhou 450002,China;UK-China Centre of Excellence for Research on Avian Diseases,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;College of Veterinary Medicine,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;College of Public Health,Zhengzhou University,Zhengzhou 450001,China;College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China)

机构地区：[1]河南省农业科学院动物免疫学重点实验室/农业农村部动物免疫学重点实验室/河南省动物免疫学重点实验室,河南郑州450002 [2]河南省农业科学院中英禽病国际研究中心,河南郑州450002 [3]河南牧业经济学院动物医药学院,河南郑州450046 [4]郑州大学公共卫生学院,河南郑州450001 [5]河南科技大学动物科技学院,河南洛阳471003

出　　处：《河南农业科学》2022年第6期134-143,共10页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(U21A20260);河南省重点研发与推广专项(212102110097);河南省自然科学基金(212300410359);河南省农业科学院自主创新项目(2022ZC65)。

摘　　要：为了解和评估现有鸡马立克病(MD)疫苗的质量,采用间接免疫荧光试验(IFA)对2020—2021年市场销售的9家不同企业生产的11个批次MD疫苗产品进行病毒效价测定和对比分析,同时用ELISA试剂盒、胶体金试纸条或RT-PCR方法分别对禽白血病病毒(ALV)和禽网状内皮组织增生症病毒(REV)的污染状况进行检测。结果表明,在所检测的MD液氮苗中,3批进口或国产的CVI988单价液氮苗之间病毒效价无显著差异,2批不同品牌814单价液氮苗效价差异显著(P<0.05),2批不同品牌双价液氮苗(CVI988+HVT或814+HVT)病毒效价差异极显著(P<0.01),但上述全部类型液氮苗产品的病毒效价均大于5 500 PFU/羽份,达到国标规定值2倍以上。然而,4批不同品牌HVT冻干苗的病毒效价均远低于产品说明书标注的2 000 PFU/羽份,仅为83.90~109.50 PFU/羽份。全部MD疫苗产品的ALV和REV检测结果均为阴性,表明这些产品洁净无污染。The effective prevention and control of Marek’s disease(MD)mainly depends on vaccine immunization. In order to determine and evaluate the quality of present MD vaccines,virus titers of 11commercial MD vaccine products provided by 9 distinct companies during 2020—2021 were determined and analyzed by indirect immunofluorescence assay(IFA). Then the potential contamination of avian leukosis virus(ALV)and reticuloendotheliosis virus(REV)was detected using commercial ELISA kits,colloidal gold test strip or RT-PCR. The results showed that for all the tested liquid nitrogen vaccines,no significant difference was observed among the three batches of imported or domestic CVI988 vaccines,while significant differences between the two 814 vaccines(P<0. 05)and two bivalent MD vaccines(CVI988+HVT or 814+HVT)(P<0. 01)were found. The virus titers of the tested liquid nitrogen MD vaccine products were all above 5 500 PFU/bird,which were more than two folds of value specified in the national standard. However,the virus titers of four freeze-dried HVT vaccine products were only about83. 90—109. 50 PFU/bird,much lower than 2 000 PFU/bird as indicated in product introductions.Contamination of ALV or REV was not detected in any of the tested MD vaccines,indicating that these vaccines were clean.

关 键 词：马立克病 疫苗 效价测定 间接免疫荧光试验 禽白血病病毒 禽网状内皮组织增生症病毒 

分 类 号：S859.797[农业科学—临床兽医学]