Differences of core genes in liver fibrosis and hepatocellular carcinoma:Evidence from integrated bioinformatics and immunohistochemical analysis  

作　　者：Yue Li Shou-Li Yuan Jing-Ya Yin Kun Yang Xin-Gang Zhou Wen Xie Qi Wang 

机构地区：[1]Department of Pathology,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China [2]Beijing Key Laboratory of Emerging Infectious Diseases,Beijing 100015,China [3]Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Beijing 100101,China [4]Center of Liver Diseases,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China

出　　处：《World Journal of Gastrointestinal Oncology》2022年第7期1265-1280,共16页世界胃肠肿瘤学杂志（英文版）（电子版）

基　　金：Supported by the Beijing Natural Science Foundation,No.7222097;Beijing Hospitals Authority the Digestive Medical Coordinated Development Center,No.XXZ0401;National Natural Science Foundation of China,No.82000555 and No.81900547;Beijing Municipal Science and Technology Commission,No.D171100003117005.

摘　　要：BACKGROUND Liver fibrosis and hepatocellular carcinoma(HCC)are common adverse consequences of chronic liver injury.The interaction of various risk factors may cause them to happen.Identification of specific biomarkers is of great significance for understanding the occurrence,development mechanisms,and determining the novel tools for diagnosis and treatment of both liver fibrosis and HCC.AIM To identify liver fibrosis-related core genes,we analyzed the differential expression pattern of core genes in liver fibrosis and HCC.METHODS Gene expression profiles of three datasets,GSE14323,GSE36411,and GSE89377,obtained from the Gene Expression Omnibus(GEO)database,were analyzed,and differentially expressed genes(DEGs)between patients with liver cirrhosis and healthy controls were identified by screening via R software packages and online tool for Venn diagrams.The WebGestalt online tool was used to identify DEGs enriched in biological processes,molecular functions,cellular components,and Kyoto Encyclopedia of Genes and Genomes pathways.The protein–protein interactions of DEGs were visualized using Cytoscape with STRING.Next,the expression pattern of core genes was analyzed using Western blot and immunohistochemistry in a carbon tetrachloride(CCl_(4))-induced liver cirrhosis mouse model and in patient liver samples.Finally,Kaplan-Meier curves were constructed using the Kaplan-Meier plotter online server.RESULTS Forty-five DEGs(43 upregulated and 2 downregulated genes)associated with liver cirrhosis were identified from three GEO datasets.Ten hub genes were identified,which were upregulated in liver cirrhosis.Western blot and immunohistochemical analyses of the three core genes,decorin(DCN),dermatopontin(DPT),and SRY-box transcription factor 9(SOX9),revealed that they were highly expressed in the CCl4-induced liver cirrhosis mouse model.The expression levels of DCN and SOX 9 were positively correlated with the degree of fibrosis,and SOX 9 level in HCC patients was significantly higher than that in fibrosis patients.Howev

关 键 词：Liver cirrhosis Hepatocellular carcinoma Bioinformatical analysis DECORIN Dermatopontin SRY-box transcription factor 9 

分 类 号：R575.2[医药卫生—消化系统] R735.7[医药卫生—内科学]