蒙药醋浸甘草对四氯化碳联合高脂饮食诱导的大鼠肝纤维化的改善作用  

Effect of Mongolian vinegar soaked licorice on liver fibrosis induced by carbon tetrachloride combined with high fat diet in rats

作　　者：萨出拉 包书茵[1] 那生桑[3] 奥乌力吉[1] SA Chu-la;BAO Shu-yin;NA Sheng-sang;AO Wu-li-ji(Inner Mongolia Minzu University,Tongliao Inner Mongolia 028000;Mongolian Medicine Research Institute of Inner Mongolia Autonomous Region,Hohhot 010020;Inner Mongolia Medical University,Hohhot 010059)

机构地区：[1]内蒙古民族大学,内蒙古通辽028000 [2]内蒙古中蒙医药研究院,呼和浩特010020 [3]内蒙古医科大学,呼和浩特010059

出　　处：《中南药学》2022年第7期1584-1589,共6页Central South Pharmacy

基　　金：内蒙古蒙药标准化项目(No.MB2019);蒙医防治优势病种经典方药研发平台及质量体系建设和示范研究(No.2018YFC1708200)。

摘　　要：目的探讨醋浸甘草对四氯化碳(CCl_(4))联合高脂饮食诱导的大鼠肝纤维化的改善作用。方法采用皮下注射40%~60%CCl_(4)橄榄油溶液(0.3 mL/100 g)联合高脂饮食,持续5周,建立大鼠肝纤维化模型。造模结束后,大鼠分为醋浸甘草低剂量(0.8 g·kg^(-1))、中剂量(2.5 g·kg^(-1))、高剂量(5 g·kg^(-1))给药组,阳性组[秋水仙碱(1.5 mg·kg^(-1))]及醋液组(2 mL·kg^(-1)),连续给药4周。通过全自动化仪检测大鼠血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转氨酶(AST)水平,通过放射免疫分析法检测血清透明质酸(HA),采用酶联免疫法(ELISA)测定血清层粘连蛋白(LN)和Ⅲ型前胶原肽(PⅢP)水平;通过HE和Masson染色法观察肝组织病理学形态变化及免疫组化法检测肝组织细胞色素CYP2E1及转录因子Nrf2表达。结果第6周时大鼠肝纤维化模型建立成功。与模型组相比,给药组的各检测指标ALT、AST、HA、LA、PⅢP及CYP2E1、Nrf2水平均有所降低(P<0.05);肝脏病理组织切片图片显示,阳性组和给药组(低、中、高)的肝纤维化明显减轻。结论醋浸甘草对四氯化碳联合高脂饮食诱导的大鼠肝纤维化具有明显改善作用,并且高剂量组与阳性组疗效相近。Objective To determine the improve effect of vinegar soaked licorice on liver fibrosis induced by carbon tetrachloride(CCl_(4))combined with high fat diet in rats.Methods Subcutaneous injection of 40%-60%CCl_(4) olive oil solution(0.3 mL/100 g)combined with a high fat diet was used for 5 weeks to establish the rat model with liver fibrosis.After the modeling,the rats were divided into a low dose(0.8 g·kg^(-1)),a medium dose(2.5 g·kg^(-1)),a high dose(5 g·kg^(-1))group,a colchicine(1.5 mg·kg^(-1))positive group,and a vinegar group(2 mL·kg^(-1)).The serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels in the rats were measured automatically.The serum hyaluronic acid(HA)was detected by radioimmunoassay,the serum laminin(LN)and procollagen typeⅢpeptide(PⅢP)levels were measured by enzyme-linked immunoassay.Liver histopathological morphological changes were observed by HE and Masson staining,and expressions of cytochrome CYP2E1 and transcription factor Nrf2 were detected by immunohistochemistry.Results The rat liver fibrosis model was established successfully at the 6th week.Compared with the model group,the levels of ALT,AST,HA,LA,PⅢP,CYP2E1 and Nrf2 of all the examined indexes in the dosing group were decreased(P<0.05).As shown in the pictures of liver pathological tissue sections,the liver fibrosis was significantly alleviated in the positive group and the 3 administration groups.Conclusion Vinegar soaked licorice can significantly improve the liver fibrosis induced by carbon tetrachloride combined with high-fat diet in rats,and the effect of the high-dose group is similar to that of the positive group.

关 键 词：蒙药 醋浸甘草 四氯化碳 高脂饮食 肝纤维化 

分 类 号：R285[医药卫生—中药学]