Structure of the myenteric plexus in normal and diseased human ileum analyzed by X-ray virtual histology slices  

作　　者：Bela Veress Niccolo Peruzzi Marina Eckermann Jasper Frohn Tim Salditt Martin Bech Bodil Ohlsson 

机构地区：[1]Department of Pathology,Skåne Universiity Hospital,Malmo 20502,Sweden [2]Medical Radiation Physics,Department of Clinical Sciences,Lund University,Lund 22100,Sweden [3]Institute for X-Ray Physics,University of Gottingen,Gottingen 37077,Germany [4]Cluster of Excellence“Multiscale Bioimaging:from Molecular Machines to Networks of Excitable Cells”(MBExC),University of Göttingen,Göttingen 37077,Germany [5]ESRF,The European Synchrotron,Grenoble 38043,France [6]Department of Internal Medicine,Skåne University Hospital,Lund University,MalmöS-20502,Sweden

出　　处：《World Journal of Gastroenterology》2022年第29期3994-4006,共13页世界胃肠病学杂志（英文版）

基　　金：Supported by the Development Foundation of Region Skane,No.REGSKANE-818781 and No.2018-Projekt0024;the Foundation Skane University Hospital,No.2020-0000028.

摘　　要：BACKGROUND The enteric nervous system(ENS)is situated along the entire gastrointestinal tract and is divided into myenteric and submucosal plexuses in the small and large intestines.The ENS consists of neurons,glial cells,and nerves assembled into ganglia,surrounded by telocytes,interstitial cells of Cajal,and connective tissue.Owing to the complex spatial organization of several interconnections with nerve fascicles,the ENS is difficult to examine in conventional histological sections of 3-5μm.AIM To examine human ileum full-thickness biopsies using X-ray phase-contrast nanotomography without prior staining to visualize the ENS.METHODS Six patients were diagnosed with gastrointestinal dysmotility and neuropathy based on routine clinical and histopathological examinations.As controls,fullthickness biopsies were collected from healthy resection ileal regions after hemicolectomy for right colon malignancy.From the paraffin blocks,4-μm thick sections were prepared and stained with hematoxylin and eosin for localization of the myenteric ganglia under a light microscope.A 1-mm punch biopsy(up to 1 cm in length)centered on the myenteric plexus was taken and placed into a Kapton®tube for mounting in the subsequent investigation.X-ray phase-contrast tomography was performed using two custom-designed laboratory setups with micrometer resolution for overview scanning.Subsequently,selected regions of interest were scanned at a synchrotron-based end-station,and high-resolution slices were reported.In total,more than 6000 virtual slices were analyzed from nine samples.RESULTS In the overview scans,the general architecture and quality of the samples were studied,and the myenteric plexus was localized.High-resolution scans revealed details,including the ganglia,interganglional nerve fascicles,and surrounding tissue.The ganglia were irregular in shape and contained neurons and glial cells.Spindle-shaped cells with very thin cellular projections could be observed on the surface of the ganglia,which appeared to build a network.

关 键 词：Enteric nervous system IMMUNOHISTOCHEMISTRY NEUROPATHY SYNCHROTRON Virtual histology Xray phase-contrast nanotomography 

分 类 号：R574[医药卫生—消化系统]