Multilocus analysis of the Exophiala jeanselmei clade containing black yeasts involved in opportunistic disease in humans  

作　　者：Jingsi Zeng Peiying Feng A.H.G.Gerrits van den Ende Liyan Xi M.J.Harrak G.S.de Hoog 

机构地区：[1]Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity Centre,P.O.Box 85167,3508 AD Utrecht,the Netherlands [2]Institute for Biodiversity and Ecosystem Dynamics,University of Amsterdam,Amsterdam,the Netherlands [3]Department of Dermatology and Venereology,Union Hospital,Tongji Medical College,Huazhong Science and Technology University,Wuhan,China [4]Third Affiliated Hospital,Sun Yat-sen University,Guangzhou,China [5]Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou,China [6]Peking University Health Science Center,Research Center for Medical Mycology,Beijing,China [7]Shanghai Institute of Medical Mycology,Changzheng Hospital,Second Military Medical University,Shanghai,China

出　　处：《Fungal Diversity》2014年第2期3-16,共14页真菌多样性（英文）

摘　　要：To confirm species delimitations in the‘jeanselmeiclade’in the Chaetothyriales,four independent markers were analysed,and phylogenetic trees were reconstructed using different algorithms.Reproductive isolation within the complex and reproductive modes in the species involved were determined,in order to explore specific borderlines using Genealogical Concordance Phylogenetic Species Recognition(GCPSR).Sequences of the Internal Transcribed Spacer(ITS),partial Translation Elongation Factor 1-α(TEF1),β-Tubulin(BT2),and Actin(ACT1)genes were analysed for a set of 81 strains.Phylogenetic reconstruction was performed using neighbour-joining(NJ),maximum parsimony(MP),maximum likelihood(ML)and Bayesian analysis(BA)to evaluate the concordance of topologies obtained under different optimization criteria.Incongruence among lineages was detected by Partition Homogeneity Test(PHT).Most Exophiala species analysed in this study seemed to be preponderantly clonal.A group of species around Exophiala spinifera showed a tendency toward human pathogenicity,whereas species around E.bergeri were often associated with environments rich in hydrocarbons.Though the tree topologies were not completely identical when different algorithms were used,the four gene lineages were nearly congruent and the trees of the combined multilocus data set did not provide more phylogenetic information than those of separate data sets.ITS data are sufficient for identification of species in the‘jeanselmei-clade’.

关 键 词：Black yeasts CLONALITY EXOPHIALA ITS diagnostics PHYLOGENY 

分 类 号：R73[医药卫生—肿瘤]