明日叶可培养内生真菌分离鉴定及功能性筛选  

Isolation,identification and functional screening of culturable endophytic fungi from Angelica keiskei

作　　者：刘雯雯 冯瑞章[1,2] 魏琴[1,2] 周万海 王虹 杜永华[1,2] LIU Wenwen;FENG Ruizhang;WEI Qin;ZHOU Wanhai;WANG Hong;DU Yonghua(Sichuan Oil Cinnamon Engineering Technology Research Center,Yibin University,Yibin 644007,China;Faculty of Agriculture,Forestry and Food Engineering,Yibin University,Yibin 644007,China)

机构地区：[1]宜宾学院四川省油樟工程技术研究中心,四川宜宾644007 [2]宜宾学院农林与食品工程学部,四川宜宾644007

出　　处：《食品与发酵工业》2022年第15期137-143,I0003,共8页Food and Fermentation Industries

基　　金：四川省科技厅项目(2018JY0495);香料植物资源开发与利用四川省高校重点实验室开放基金项目(2015XLZ004);宜宾学院科研创新团队项目(2017TD01,2018TD04)。

摘　　要：为揭示明日叶内生真菌的多样性及筛选可利用菌株,利用选择性平板分离培养技术,对明日叶茎、叶、花和果实器官中可培养内生真菌进行分离,结合ITS序列和系统发育树分析菌株多样性,并筛选产黄酮和抑菌功能菌株。明日叶中共分离出34株可培养内生真菌,可划分为1门、4纲、8目、10科、10属、22种,优势属为Cladosporium(32.4%)。以纲为类群单位,果实器官的5属8种12株系统发育可分为4个类群,茎的5属10种11株分为3个类群,叶的5属8种9株分为2个类群,花的1属2种2株分为1个类群。果实中的优势菌为Alternaria alternata,茎的为Cladosporium tenuissimum,叶的为Colletotrichum karsti,花的为Aspergillus sp.。对产黄酮和抑菌功能的筛选发现,9株产黄酮,15株对至少1种病原细菌有抑制效果,26株对至少1种真菌指示菌显示抑制作用,其中菌株MRY-1效能最佳,最优培养基条件为初始pH 7、时间7 d、温度26℃、转速120 r/min、装液量60 mL/150 mL。因此,明日叶不同器官中分离的内生真菌具有遗传多样性,筛选得到的功能性菌株值得深入挖掘和开发利用。To reveal the diversity of endophytic fungi in Angelica keiskei and screen the strains available,the culturable endophytic fungi in the stems,leaves,flowers and fruit of A.keiskei were isolated using selective media.The diversity of strains was analyzed using their ITS sequences and a phylogenetic tree.The flavonoids that were produced,and the antibacterial strains were screened.A total of 34 strains of culturable endophytic fungi were isolated,and they could be divided into one phylum,four classes,eight orders,10 families,10 genera and 22 species.The dominant genus was Cladosporium sp.(32.4%).Using the class as a group unit,the phylogeny of fruit included five genera,eight species and 12 plants that could be divided into four groups.The stems contained five genera and 10 species.Eleven plants could be divided into three groups.The leaves contained five genera,eight species,and nine plants that could be divided into two groups.The flowers contained one genus,two species,and two plants that could be divided into one group.The dominant species on the fruit was Alternaria alternata.Those on the stems were Cladosporium tenuissimum;the leaves contained Colletotrichum karsti,and the flowers contained species of Aspergillus.The screening of flavonoid production and antibacterial properties indicated that nine strains produced flavonoids.Fifteen strains inhibited at least one pathogenic bacterium,and 26 strains inhibited at least one fungal indicator.Among them,strain MRY-1 was the most effective.The optimal conditions of media included an initial pH of 7,7 d,26℃,and a rotation speed of 120 r/min.The liquid loaded included 60 mL/150 mL.Therefore,the endophytic fungi isolated from different organs of A.keiskei are genetically diverse,and the functional strains that were screened are worthy of further excavation,development and utilization.

关 键 词：明日叶 内生真菌 分子鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]