肺结节组织中Caspase 3和Caspase9蛋白表达在良恶性诊断及肺癌临床病理特征中的价值研究  

Value of Caspase3 and Caspase9 Protein Expression in Lung Nodules in Diagnosis of Benign and Malignant and Clinicopathological Characteristics of Lung Cancer

作　　者：陈敬信 王小玲 CHEN Jing-xin;WANG Xiao-ling(Department of Pathology,Shaanxi Sengong Hospital,Xi’an 710300,China;Department of Pathology,the Second Hospital of Weinan,Shaanxi Weinan 714000,China)

机构地区：[1]陕西省森工医院病理科,西安710300 [2]渭南市第二医院病理科,陕西渭南714000

出　　处：《现代检验医学杂志》2022年第4期92-95,共4页Journal of Modern Laboratory Medicine

摘　　要：目的探讨肺结节组织中半胱氨酸天冬氨酸蛋白酶(cysteine aspastic acid-specific protease,Caspase)3,半胱氨酸天冬氨酸蛋白酶(cysteine aspastic acid-specific protease,Caspase)9蛋白表达在其良恶性诊断及肺癌临床病理特征中的价值研究。方法选择2018年1月~2020年7月在陕西省森工医院诊治的86例肺结节患者作为研究对象,并根据病理诊断结果分为良性结节46例(良性组)和恶性结节40例(恶性组)。然后采用免疫组织化学法检测两组患者肺结节组织中Caspase 3,Caspase 9蛋白表达情况,调查恶性肺结节的病理特征,并进一步分析Caspase 3和Caspase 9表达阳性率与病理特征的相关性。结果恶性组的Caspase 3和Caspase 9表达阳性率分别为70.0%和82.5%,显著高于对照组(13.0%,15.2%),差异均有统计学意义(χ^(2)=29.035,38.932,均P<0.001)。在恶性组中,不同组织学分化、淋巴结转移、临床分期患者的Caspase 3,Caspase 9表达阳性率对比差异有统计学意义(χ^(2)=5.614~15.920,均P<0.05)。Pearson相关性分析显示组织学分化、淋巴结转移、临床分期与Caspase 3,Caspase 9表达阳性率均呈显著正相关(r=0.591~0.762,均P<0.05)。结论肺结节组织中Caspase 3,Caspase 9蛋白呈现高表达状况,也与患者的病理特征存在相关性,因此其临床检测有利于肺结节良恶性的临床鉴别诊断。Objective To investigate the value of Caspase3 and Caspase9 protein expression in lung nodules in the diagnosis of benign and malignant and the clinical pathological characteristics of lung cancer.Methods 86 patients with pulmonary nodules who were diagnosed and treated in Shaanxi Sengong Hospital from January 2018 to July 2020 were selected as the research objects.And according to the pathological diagnosis results,they were divided into 46 cases of benign nodules(benign group)and 40 cases of malignant nodules(malignant group).Then immunohistochemical method was used to detect the expression of Caspase3 and Caspase9 protein in the lung nodules of the two groups of patients.The pathological characteristics of malignant lung nodules were investigated and the correlation between the positive rate of Caspase3 and Caspase9 expression and pathological characteristics was further analyzed.Results There were 46 cases were pathologically diagnosed as benign nodules(benign group)and 40 cases were malignant nodules(malignant group).The positive rates of Caspase3 and Caspase9 expression in the malignant group were 70.0%and 82.5%,respectively,which were higher than 13.0%and 15.2%in the control group,the differentes were statistically significant(χ^(2)=29.035,38.932,all P<0.001).In the malignant group,the positive rates of Caspase3 and Caspase9 expression in patients with different histological differentiation,lymph node metastasis,and clinical stages were significantly different(χ^(2)=5.614~15.920,all P<0.05).Pearson correlation analysis showed that histological differentiation,lymph node metastasis,clinical staging were significantly positively correlated with the positive rates of Caspase3 and Caspase9(r=0.591~0.762,all P<0.05).Conclusion The high expression of Caspase3 and Caspase9 proteins in lung nodule tissue was also related to the pathological characteristics of the patient.Therefore,their clinical detection is beneficial to the clinical differential diagnosis of benign and malignant pulmonary nodules.

关 键 词：肺结节 良恶性 半胱氨酸天冬氨酸蛋白酶3 半胱氨酸天冬氨酸蛋白酶9 诊断价值 病理特征 

分 类 号：R734.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]