Effect of Peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition  

作　　者：HUOGEN LIU YUNDI SHI XIN WAN YING LIU HAILIN SHU FENGMING HUANG ZHENBIN GONG LING GU 

机构地区：[1]Department of Critical Care Medicine,Mindong Hospital Affiliated to Fujian Medical University,Ningde,355000,China

出　　处：《BIOCELL》2022年第12期2671-2680,共10页生物细胞（英文）

基　　金：The study was supported by the Fujian Provincial Science and Technology Department of the Project Fund under Grant(Grant No.2015J01573);the Medical Innovation Projects of Fujian Province’s Health and Scientific Research Talent Training Project under Grant(Grant No.2019-CXB-25).

摘　　要：Peroxiredoxin 1(PRDX1)participates in tumor cell proliferation,apoptosis,migration,invasion,and the epithelial-to-mesenchymal transition(EMT).This study aimed to investigate the effect of PRDX1 on the EMT of airway epithelial cells stimulated with lipopolysaccharide(LPS)and transforming growth factor-beta 1(TGF-β1).PRDX1 overexpression significantly increased the proliferation and migration of human bronchial epithelial(BEAS-2B)cells,reduced cell apoptosis(p<0.01),and induced EMT and collagen deposition by upregulating the expression of the matrix metallopeptidase(MMP)2,MMP9,α-smooth muscle actin(α-SMA),N-cadherin,vimentin and twist proteins and inhibiting E-cadherin expression(p<0.05).PRDX1 overexpression promoted TGF-β1-mediated inhibition of cell proliferation and migration and significantly enhanced the TGF-β1-induced EMT and collagen synthesis(p<0.05).Knockdown of PRDX1 inhibited cell proliferation,migration,EMT,and collagen synthesis(p<0.01),reversed LPS-mediated inhibition of cell proliferation and migration,and significantly suppressed LPS-induced EMT and collagen synthesis(p<0.01).The result indicating that PRDX1 may be involved in LPS/TGF-1-induced EMT and collagen synthesis in human bronchial epithelial cells.

关 键 词：Peroxiredoxin 1 Airway epithelial cell Epithelial-to-mesenchymal transition Cell migration Collagen synthesis 

分 类 号：R73[医药卫生—肿瘤]