废弃稀土尾砂地先锋植物根区核心菌群研究  

Root Core Microbiome of Pioneer Plants in Abandoned Rare Earth Mine Tailings

作　　者：彭钰欣 刘叶 陈子武 刘文深 王诗忠[1,2] 汤叶涛[1,2] 仇荣亮[1,2,3,4] 晁元卿 PENG Yuxin;LIU Ye;CHEN Ziwu;LIU Wenshen;WANG Shizhong;TANG Yetao;QIU Rongliang;CHAO Yuanqing(School of Environmental Science and Engineering,Sun Yat-sen University,Guangzhou 510006,China;Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology,Guangzhou 510006,China;Guangdong Provincial Key Laboratory of Agricultural&Rural Pollution Abatement and Environmental Safety,College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510642,China;Guangdong Laboratory for Lingnan Modem Agriculture,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]中山大学环境科学与工程学院,广东广州510006 [2]广东省环境污染控制与修复技术重点实验室,广东广州510006 [3]华南农业大学资源环境学院,广东省农业农村污染治理与环境安全重点实验室,广东广州510642 [4]华南农业大学,岭南现代农业科学与技术广东省实验室,广东广州510642

出　　处：《环境科学与技术》2022年第4期173-183,共11页Environmental Science & Technology

基　　金：国家重点研发计划重点专项(2020YFC1807600);国家自然科学基金面上项目(42077105,41771343);广州市科技计划项目(202002030333)。

摘　　要：为探究稀土矿区废弃尾砂地胁迫生境下先锋植物根相关微生物组的群落结构和集群模式,该文采集废弃3年、6年、10年的稀土尾矿土壤及典型先锋植物芒萁和芒草根样品,利用16S rRNA高通量测序技术对先锋植物根相关微生物组进行测序和生物信息学分析。结果表明,自然演替下芒萁和芒草根区形成了以高耐性菌和植物促生菌为主的核心微生物,如杆菌科、蓝藻科、伯克氏菌科、鱼孢菌科等;根部分区导致芒萁根际与非根际土壤的微生物群落组成存在较大差异,但芒草的较为相似;芒萁根内富集菌群与稀土高度相关,而芒草的与金属耐性相关;2种植物根区均出现了甲基营养型菌属和与营养获取相关的基石物种,可能是芒萁和芒草成为废弃稀土尾砂地先锋植物的关键。In order to explore the community structure and cluster mode of root-associated microbiomes of pioneer plants in the stressed habitat at abandoned rare earth mine tailings,soil samples were collected from the rare earth mine tailings discarded for 3,6 and 10 years,respectively.The samples of typical pioneer plants Dicranopteris dichotoma and Miscanthus sinensis roots were also collected.16S rRNA high-throughput sequencing technology was employed to sequence and analyze the bioinformatics of the gene V4 variable region.Results showed that under natural succession,associated with D.dichotoma and M.sinensis root,core microorganisms were dominated by high tolerant bacteria and plant growth promoting bacteria,such as Ilumatobacteraceae,Cyanobiaceae,Burkholderiaceae and Sporichthyaceae.The root area led to a large difference in the microbial community composition between the rhizosphere and bulk soil of D.dichotoma,but the microbial community composition of M.sinensis was more similar.The enriched microbes in D.dichotoma root were highly correlated with rare earth elements,while the enriched microbes in M.sinensis root were more related to metal tolerance.Methylotrophic bacteria and cornerstone species related to nutrient acquisition appeared in the root of both plants,which may be the key that D.dichotoma and M.sinensis became the pioneer plants in abandoned rare earth mine tailings.

关 键 词：稀土尾砂地 先锋植物 芒萁 芒草 根相关微生物组 核心菌群 

分 类 号：X172[环境科学与工程—环境科学]