Characterization of three-dimensional multipotent adipose-derived stem cell spheroids  

作　　者：HONGYANG LI CHAN WANG SHIWEI LIU YONGLONG GUO JIANSU CHEN 

机构地区：[1]Ophthalmology Department,First Affiliated Hospital of Jinan University,Guangzhou,510632,China [2]Ophthalmology Department,Guangdong Second Provincial General Hospital,Guangzhou,510310,China [3]Institute of Ophthalmology,Medical College,Jinan University,Guangzhou,510632,China [4]Key Laboratory for Regenerative Medicine of Ministry of Education,Jinan University,Guangzhou,510632,China

出　　处：《BIOCELL》2022年第7期1705-1716,共12页生物细胞（英文）

基　　金：This study was supported by the National Natural Science Foundation of China(21318261 and 3201101202 to JC);National Natural Science Foundation of China(82000943 to YG);the Joint Fund of Basic and Applied Basic Research Fund of Guangdong Province(2019A1515110355 to YG);the Project funded by China Postdoctoral Science Foundation(2019M663391 and 2021T140273 to YG).

摘　　要：Human adipose stem cells(hADSCs)are reliable sources for cell therapy.However,the clinical applications are limited by the decrease in activity during in vitro culture.We used a knockout serum replacement(KSR)medium,Eppendorf(EP)tube culture,and a simulated microgravity(SMG)culture system to establish hADSC spheroids.We found that hADSCs aggregated and formed spheroids in the KSR culture medium.The EP tube culture method revealed many biological cell characteristics,such as good cell viabilities,rough surfaces,polar growth,fusion phenomenon,and injectability.The findings show its advantages for hADSCs spherical cultures.When cultured in SMG,hADSC spheroids produced large-scale spheroids.Additionally,confocal examination and viability assays revealed that SMG-cultured hADSC spheroids had higher cell viabilities and looser spherical structures,relative to those cultured in EP tubes.hADSC spheroids in static EP tube culture had tighter structures and more dead cells with rough and irregular surfaces,while hADSC spheroids in dynamic SMG condition exhibited looser structures and better cell viabilities with flat and regular surfaces.Therefore,the KSR media promotes spherical formation by hADSCs,which showed polar growth,fusion,and injectability in vitro.The dynamic SMG culture enhances the formation of a looser structure and better cell viabilities for hADSC spheroids.

关 键 词：hADSCs EP tube SPHEROIDS MICROGRAVITY 

分 类 号：O43[机械工程—光学工程]