Offspring from oocytes grown in frozen-thawed ovarian tissues transplanted to male and female bodies  

作　　者：Jie Yan Yu-Fang Cai Shan-Jun Dai Xiao-Jin He Ying-Pu Sun Yun-Xia Cao Jie Qiao Ri-Cheng Chian 

机构地区：[1]Center for Reproductive Medicine,Peking University Third Hospital,Beijing 100191,China [2]Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education,School of Basic Medicine,Ningxia Medical University,Yinchuan 750004,China [3]Center for Reproductive Medicine,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China [4]Center for Reproductive Medicine,Anhui Medical University,Hefei 230032,China [5]Center for Reproductive Medicine,Shanghai 10th People's Hospital of Tongji University,Shanghai 200072,China

出　　处：《Reproductive and Developmental Medicine》2022年第1期13-19,共7页生殖与发育医学（英文版）

基　　金：Financial support and sponsorship:J.Y.was sponsored by Peking University Third Hospital Beijing,China;Y.C.was sponsored by Ningxia Medical College,Yinchuan,China;S.D.was sponsored by The First Affiliated Hospital of Zhengzhou University,Zhengzhou,China;X.H.was sponsored by Anhui Medical University,Hefei,China。

摘　　要：Objective: There are few reports of live births from heterotopic transplantation of frozen-thawed ovarian tissue. The purpose of this study is to assess the follicular development in the frozen-thawed ovarian tissues following heterotopic transplantation in both female and male bodies.Methods: Cluster of differentiation 1 (CD1) mice (6-8 weeks) were used in this study as ovarian tissue donors and foster mothers for embryo transfer. Sperm from CD1 male mice were used for insemination by intracytoplasmic sperm injection (ICSI). Nude severe combined immunodeficiency mice (8 weeks) were employed as recipients of ovarian tissue transplantation. The frozen-thawed ovarian tissues were transplanted to 4 sites on each mouse, female and male, subcutaneously. After 3 months, both female and male mice were injected with 5.0 IU gonadotropins intraperitoneally. Post 48 hours of injection, the mouse was killed for ovarian transplant collection. Only fully grown oocytes with contacted cumulus cells (cumulus-oocyte complexes) were then selected for maturationin vitro.In vitro matured oocytes were inseminated with fresh sperm by ICSI, and the developed blastocysts were frozen using the vitrification method and stored until embryo transfer. After thawing, the thawed blastocysts were incubated for at least 2 hours before the transfer. The foster mice mothers mated with vasectomised male 3 days previously. Live birth was monitored at 19 days after transfer, and the resulted offspring was raised for fertility test.Results: The relatively high recovery rates of the transplanted ovarian tissues were collected in both frozen-thawed and fresh ovarian tissue transplants from both female and male bodies. The fully grown immature oocytes became maturein vitro and the fertilized zygotes developed to blastocyst stage. There are no differences between frozen-thawed and fresh ovarian transplants in term of oocyte quality and embryo development to blastocyst rates. Nineteen-day post-transfer, 3 foster mothers from the frozen-thawed ovarian tissue

关 键 词：Ovarian tissue CRYOPRESERVATION TRANSPLANTATION OOCYTES Live births 

分 类 号：R714.8[医药卫生—妇产科学]