两种提取法的枇杷果肉萜类化合物组分含量差异分析  

Difference Analysis on the Compounds and Contents of Terpenoids of Loquat Fresh between Two Extraction Methods

作　　者：陈秀萍[1] 苏文炳 蒋际谋[1] 姜帆[1] 陈永萍 CHEN Xiu-ping;SU Wen-bing;JIANG Ji-mou;JIANG Fan;CHEN Yong-ping(Fruit Research Institute,Fujian Academy of Agricultural Sciences/Fujian Breeding Engineering Technology Research Center for Longan&Loquat,Fuzhou,Fujian 350013,China)

机构地区：[1]福建省农业科学院果树研究所/福建省龙眼枇杷育种工程技术研究中心,福建福州350013

出　　处：《东南园艺》2022年第2期99-106,共8页Southeast Horticulture

基　　金：福建省属公益类科研院所基本科研专项(2019R1028-4);科技部、财政部国家科技资源共享服务平台项目(NHGRC2021-NH18-1);农业高质量发展超越“5511”协同创新工程(XTCXGC2021006);福建省农业科学院科技创新团队(CXTD2021004-1);国家科技计划项目(2021L3022);福建省农业科学院对外合作项目(DWHZ2021-14);福建省农业种质资源创新专项(ZZZYCXZX202207)。

摘　　要：本研究采用两种方法提取‘Marc’枇杷果肉中的萜类化合物,并利用超高效液相色谱-串联质谱技术分析鉴定提取物中的萜类化合物组分及峰面积。结果表明,方法一(无水乙醇+超声提取、浓缩+甲醇溶解+70%甲醇+涡旋)的提取物中鉴定到萜类化合物39种,方法二(70%甲醇+涡旋)的提取物中鉴定到萜类化合物15种;两者共有化合物相对含量也存在差异,方法一比方法二提取枇杷果肉萜类化合物的效果更好,且提取出的相对含量高出11.8~943.3倍。In this study,two different methods were used to extract the terpenoids from the fresh of Eriobotrya japonica Lindl.cv Marc,and UPLC-MS/MS was used to determine the compounds of terpenoids and their peak areas.The results showed that a total of 39 compounds of terpenoids were identified using the first method with anhydrous ethanol,ultrasonic extraction,concentration methanol dissolution and 70%methanol combined with vortex.A total of 15 compounds of terpenoids were identified using the second method with 70%methanol combined with vortex.The relative contents of common substances between the two methods were also different.The effect using the first method was better than that using the second method and the relative content using the first method was 11.8~943.3 times higher than using the second method.

关 键 词：枇杷 果肉 萜类 提取法 超高效液相色谱-串联质谱(UPLC-MS/MS) 

分 类 号：S667.3[农业科学—果树学]