鼻内翻性乳头状瘤中HPV感染状况分析  

Analysis of HPV infection in nasal inverted papilloma

作　　者：谢佳[1] 颜永毅[1] 龚辉成[1] Xie Jia;Yan Yongyi;Gong Huicheng(Guangzhou Twelfth People’s Hospital,Guangzhou Otolaryngology Head and Neck Surgery Hospital,Guangzhou 510620,China)

机构地区：[1]广州市第十二人民医院,广州市耳鼻咽喉头颈外科医院耳鼻咽喉头颈外科,广州510620

出　　处：《新医学》2022年第8期608-611,共4页Journal of New Medicine

基　　金：广东省医学科学技术研究基金(B2019141)。

摘　　要：目的 分析鼻内翻性乳头状瘤(NIP)中人乳头状瘤病毒(HPV)感染的情况,为进一步研究HPV介导NIP发病、复发及恶变机制提供实验及理论依据。方法 应用核酸分子快速导流杂交基因芯片分型技术(HybriMax)检测NIP实验组(48例)及正常鼻黏膜对照组(20例)中HPV的感染率及其各亚型的表达情况。结果 NIP实验组组织标本中HPV总阳性率为64.58%(31/48),正常鼻黏膜对照组组织标本中HPV检测结果均为阴性,2组阳性率比较差异有统计学意义(χ^(2)=32.178,P <0.01)。在31例HPV阳性标本中共检测出HPV病毒49株,含12个亚型,其中HPV6占4.08%(2/49)、HPV11占32.65%(16/49)、HPV16占12.24%(6/49)、HPV18占4.08%(2/49)、HPV33占4.08%(2/49)、HPV39占2.04%(1/49)、HPV44占10.20%(5/49)、HPV52占8.16%(4/49)、HPV53占2.04%(1/49)、HPV56占2.04%(1/49)、HPV58占16.30%(8/49)、HPV68占2.04%(1/49)。结论 HPV感染与NIP的发病有着密切关系,国内NIP的发生发展可能与HPV11、16、44、58亚型有关。Objective To analyze the infection of human papilloma virus(HPV)in nasal inverted papilloma(NIP)through experiments,aiming to provide experimental and theoretical basis for further research on the incidence,recurrence and malignant transformation mechanism of HPV-mediated NIP.Methods The infection rates and expression levels of HPV subtypes in the experimental group(NIP group,n=48)and control group(normal nasal mucosa,n=20)were detected by nucleic acid molecular rapid diversion hybrid gene chip typing technique(HybriMax).Results The total positive rate of HPV in the tissue samples of the NIP group was 64.58%(31/48).The HPV test results of normal nasal mucosa specimens in the control group were all negative,and the difference between two groups was statistically significant(χ^(2)=32.178,P<0.01).Forty-nine strains of HPV virus were detected in 31 HPV positive specimens.A total of 12 subtypes were classified including 4.08%(2/49)of HPV6,32.65%(16/49)of HPV11,12.24%(6/49)of HPV16,4.08%(2/49)of HPV18,4.08%(2/49)of HPV33,2.04%(1/49)of HPV39,10.20%(5/49)of HPV44,8.16%(4/49)of HPV52,2.04%(1/49)of HPV53,2.04%(1/49)of HPV56,16.30%(8/49)of HPV58,and 2.04%(1/49)of HPV68.Conclusion HPV infection is closely associated with the incidence of NIP,and the occurrence and development of NIP in China may be correlated with HPV11,16,44 and 58 subtypes.

关 键 词：人乳头状瘤病毒 鼻内翻性乳头状瘤 亚型 感染率 复发 

分 类 号：R739.62[医药卫生—肿瘤]