New Promises of Advanced Molecular Recognition:Bioassays,Single Cell Analysis,Cancer Therapy,and Beyond  

作　　者：Shuxin Xu Hui He Zhen Liu 

机构地区：[1]State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,163 Xianlin Avenue,Nanjing,Jiangsu,210023 China

出　　处：《Chinese Journal of Chemistry》2022年第5期635-650,共16页中国化学（英文版）

基　　金：We acknowledge financial suport of the National Science Fund for Distinguished Young Scholars(No.21425520)and the Key Grant(No.21834003)from the National Natural Science Foundation of China;the National Key R&D Program of China(No.2018YFC0910301)from the Ministry of Science and Technology of China;the Excellent Research Program of Nanjing University(No.ZYJH004).

摘　　要：The development of biomimetic affinity materials holds great value for scientific research and practical applications.Starting from boronic acid chemistry,we developed a series of boronate affinity materials(BAMs)with desired binding properties in aspects of binding pH,affinity and selectivity.Based on BAMs,molecularly imprinted polymers(MIPs)and aptamers capable of targeting vital biomolecules were rationally designed and prepared.Using these affinity reagents as potent tools and combining with advanced analytical techniques particularly liquid chromatography,mass spectrometry and spectrometry allowed for the construction of promising analytical methods to provide new qualitative and quantitative solutions to challenging applications including targeted proteomics analysis,single cell analysis and disease diagnostics.In addition,the targeting capability of these affinity reagents particularly nanoscale MIPs opened a unique access to develop innovative nanotherapeutics for cancer treatment.

关 键 词：Molecular recognition Boronate afinity Molecularly imprinted polymers Immunoassays CANCER 

分 类 号：Q2-33[生物学—细胞生物学]