An endoparasitoid uses its egg surface proteins to regulate its host immune response  

作　　者：Zi-Wen Teng Hui-Zi Wu Xin-Hai Ye Qi Fang Hong-Xu Zhou Gong-Yin Ye 

机构地区：[1]China-Australia Cooperation Base of Crop Health and Invasive Species,China-Australia Joint Institute of Agricultural and Environmental Health,College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao,Shandong Province,China [2]State Key Laboratory of Rice Biology,Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects,Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Institute of Insect Sciences,Zhejiang University,Hangzhou,China

出　　处：《Insect Science》2022年第4期1030-1046,共17页昆虫科学（英文版）

基　　金：Financial support was provided by National Natural Science Foundation of China(Grant No.31801796,http:/www.nsfc.gov.cn/);Major International(Regional)Joint Research Project of National Natural Science Foundation(Grant No.31620103915,http://www.nsfc.gov.cn/);Key Program of National Natural Science Foundation of China(NSFC)(Grant no.31830074,http:/www.nsfc.gow.cn/);National Key R&D Program of China(No.2017YFD0200400);The Program for Chi-nese Innovation Team in Key Areas of Science and Technology of Ministry of Science and Technology ofthe People's Republic of China(2016RA4008);Program for Chinese Outstanding Talents in Agricultural Scientifie Research of Ministry of Agriculture and Rural Affairs of the People's Republic of China.

摘　　要：With proteomic analysis,we identified 379 egg surface proteins from an endoparasitoid,Cotesia chilonis.Proteins containing conserved enzymatic domains constitute a large proportion of egg surface components.Some proteins,such as superoxidase dismutase,homolog of C.rubecula 32-kDa protein,and immunoevasive protein-2A,are classical parasitism factors that have known functions in host immunity regulation.Melanization assays revealed that a novel egg surface protein,C.chilonis egg surface serpin domain-containing protein had the same function as a C.chilonis venom serpin,as both suppressed host melanization in a dose-dependent manner.C.chilonis egg surface serpin domain-containing protein is mainly transcribed in C.chilonis oocytes with follicular cells,and it is located on both the anterior and posterior sides of the mature egg surface.Additionally,we used LC-MS/MS to identify 586 binding proteins sourced from C.suppressalis plasma located on the eggshell surface of C.chilonis,which included some immunity-related proteins.These results not only indicate that C.chilonis uses its egg surface proteins to reduce the immune response of its host but also imply that endoparasitoid egg surface proteins might be a new parasitism factor involved in host immune regulation.

关 键 词：egg surface protein immune regulation MELANIZATION parasitism factor parasitoid wasp 

分 类 号：Q96[生物学—昆虫学]