高压氧增强宫颈癌Hela细胞对顺铂药物敏感性的研究  

Study on hyperbaric oxygen enhancing the sensitivity of cervical cancer cells(Hela)to cisplatin

作　　者：王丽平[1] 梁燕 许占英[1] 赵静[1] 李新丽 Liping Wang;Yan Liang;Zhanying Xu;Jing Zhao;Xinli Li(Department of Gynecology&Obstetrics,West Branch of the Second Hospital of Shanxi Medical University,Taiyuan 030053,China;Department of Gynecology&Obstetrics,PLA No.322 Hospital,Datong 037006,China)

机构地区：[1]山西医科大学第二医院西院妇产科,太原030053 [2]解放军第三二二医院妇产科,大同037006

出　　处：《中华航海医学与高气压医学杂志》2022年第3期308-311,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的:探讨高压氧(HBO)增强宫颈癌Hela细胞对顺铂(DDP)化疗的敏感性及其作用机制。方法:宫颈癌Hela细胞培养完成后,根据实验设计将细胞分成对照组、DDP组、HBO组和HBO+DDP组。对照组不作任何处理,DDP组采用20 μmol/L DDP处理24 h,HBO组采用0.2 MPa HBO处理3 h,HBO+DDP组在HBO预处理3 h后加入20 μmol/L DDP处理24 h。采用CCK-8法检测Hela细胞增殖能力,流式细胞术检测Hela细胞凋亡情况,Transwell实验检测Hela细胞迁移能力,Western blotting实验检测Hela细胞凋亡相关蛋白的表达水平。结果:与对照组和HBO组比较,DDP组Hela细胞存活率明显降低( P<0.05),HBO+DDP组Hela细胞存活率明显低于DDP组( P<0.05)。HBO+DDP组Hela细胞迁移率[(19.61±1.53)%]明显低于DDP组[(48.19±2.47)%],差异有统计学意义( P<0.05)。HBO+DDP组Hela细胞凋亡率[(58.68±3.69)%]明显高于DDP组[(35.74±3.57)%],差异有统计学意义( P<0.05)。HBO+DDP组Hela细胞中Bad、caspase-3蛋白表达水平较DDP组明显升高,Bcl-2蛋白表达明显降低,差异均有统计学意义( P<0.05或 P<0.01)。 结论:HBO与DDP联用通过提高DDP对Hela细胞的增殖抑制作用、诱导凋亡作用及迁移阻滞作用,提高宫颈癌Hela细胞对DDP的敏感性。Objective To discuss whether hyperbaric oxygen(HBO)can enhance the drug sensitivity of Hela cells to cisplatin(DDP)and how does it work.Methods After the Hela cells were cultured,they were divided into control group,DDP group,HBO group,and HBO+DDP group.The control group was not treated.The DDP group was treated with 20μmol/L DDP for 24 h.The HBO group was treated with 0.2 MPa HBO for 3 h,and the HBO+DDP group was treated with 20μmol/L DDP for 24 h after HBO pretreatment for 3 h.The proliferation of Hela cells was detected by CCK-8 assay,and the apoptosis of Hela cells was detected by flow cytometry.Transwell assay was used to detect the migration ability of Hela cells,and western blotting was used to detect the expression levels of proteins related to the apoptosis of Hela cells.Results The Hela cells survival rate in the DDP group was significantly lower than those in the control group and the HBO group,and was higher than that in the HBO+DDP group(P<0.05).The migration rate of Hela cells in the HBO+DDP group[(19.61±1.53)%]was significantly lower than that in the DDP group[(48.19±2.47)%],and the difference was statistically significant(P<0.05).The apoptosis rate of Hela cells in the HBO+DDP group[(58.68±3.69)%]was significantly higher than that in the DDP group[(35.74±3.57)%],and the difference was statistically significant(P<0.05).The protein expression levels of Bad and caspase-3 in Hela cells in the HBO+DDP group were significantly increased and the protein expression of Bcl-2 was significantly decreased compared with those in the DDP group,and the differences were all statistically significant(P<0.05 or P<0.01).Conclusion The combination of HBO and DDP can improve the sensitivity of Hela cells to cisplatin chemotherapy by enhancing the proliferation inhibition,apoptosis-inducing,and migration-blocking effects of DDP on Hela cells.

关 键 词：高压氧 宫颈癌 HELA细胞 耐药性 

分 类 号：R737.33[医药卫生—肿瘤]