HBB在肺癌组织中的表达及临床意义  

Expression and clinical significance of HBB in lung cancer tissue

作　　者：徐鑫鑫[1] 蔡花[1] 彭静静 刘红利[3] 褚福营[2] XU Xinxin;CAI Hua;PENG Jingjing;LIU Hongli;CHU Fuying(Department of Clinical Laboratory,Affiliated Hospital of Nantong University,Nantong,Jiangsu 226000,China;Department of Clinical Laboratory,Nantong First People′s Hospital,Nantong,Jiangsu 226000,China;Department of Clinical Laboratory,Nantong Tumor Hospital,Nantong,Jiangsu 226000,China)

机构地区：[1]南通大学附属医院检验科,江苏南通226000 [2]江苏省南通市第一人民医院检验科,江苏南通226000 [3]江苏省南通市肿瘤医院检验科,江苏南通226000

出　　处：《检验医学与临床》2022年第16期2181-2184,共4页Laboratory Medicine and Clinic

基　　金：江苏省南通市科技项目(JC2020070)。

摘　　要：目的探讨人血红蛋白β(HBB)在肺癌组织中的表达及临床意义。方法收集南通市肿瘤医院收治的56例肺癌患者的肺癌组织和癌旁组织标本,采用实时荧光定量PCR(qRT-PCR)检测HBB的表达水平,并分析其与肺癌临床病理特征之间的关系;采用受试者工作特征曲线评价HBB对肺癌的诊断价值;采用Kaplan-Meier Plotter数据库分析HBB与肺癌预后的关系。结果肺癌组织中HBB表达水平明显低于癌旁组织,差异有统计学意义(P<0.05)。肺癌组织中HBB高表达12例,低表达44例。肺癌组织中HBB的表达水平与患者性别、TNM分期、5年生存情况有关(P<0.05),而与年龄、吸烟史、病理类型、肿瘤最大径及是否有淋巴结转移无关(P>0.05)。HBB诊断肺癌的曲线下面积为0.714(95%CI:0.648~0.834),当最佳截断值为11.91时,其诊断灵敏度为71.4%,特异度为69.6%。Kaplan-Meier生存分析发现,HBB低表达患者生存率明显低于高表达患者(HR=0.77,95%CI:0.67~0.89,P<0.05)。结论HBB在肺癌组织中低表达,可能参与了肺癌的发生、发展,且对肺癌具有一定的临床诊断价值。Objective To investigate the expression and clinical significance of human hemoglobinβ(HBB)in lung cancer tissue.Methods The lung cancer tissue and adjacent tissue samples of 56 lung cancer patients treated in Nantong Tumor Hospital were collected,and the expression level of HBB was detected by real-time fluorescent quantitative PCR(qRT-PCR).And analyzed the relationship between the expression level of HBB and the clinicopathological characteristics of lung cancer.The receiver operating characteristic curve was used to evaluate the diagnostic value of HBB in lung cancer.The Kaplan-Meier Plotter database was used to analyze the relationship between HBB and the prognosis of lung cancer.Results The expression level of HBB in lung cancer tissue was significantly lower than that in adjacent tissue,and the difference was statistically significant(P<0.05).There were 12 cases of high HBB expression and 44 cases of low HBB expression in lung cancer tissues.The expression level of HBB in lung cancer tissue was related to the patient′s gender,TNM stage,and 5-year survival(P<0.05),but was not related to age,smoking history,pathological type,maximum tumor diameter and lymph node metastasis or not(P>0.05).The area under the curve of HBB for the diagnosis of lung cancer was 0.714(95%CI:0.648—0.834).When the optimal cut-off value was 11.91,the diagnostic sensitivity was 71.4%,and the specificity was 69.6%.Kaplan-Meier survival analysis showed that the survival rate of patients with low HBB expression was significantly lower than that of patients with high HBB expression(HR=0.77,95%CI:0.67—0.89,P<0.05).Conclusion HBB is lowly expressed in lung cancer tissue,may be involved in the occurrence and development of lung cancer,and has certain clinical diagnostic value for lung cancer.

关 键 词：人血红蛋白β 肺癌 癌旁组织 生存分析 病理特征 

分 类 号：R734.2[医药卫生—肿瘤]