甲状腺癌组织中DGCR8与DICER1基因表达及临床意义  

Gene expression and clinical significance of DGCR8 and DICER1 in thyroid cancer tissue

作　　者：张莉[1] 王亮[2] 王俊[1] 程艳荣 王琳[2] Zhang Li;Wang Liang;Wang Jun;Cheng Yanrong;Wang Lin(Department of Laboratory,Xi'an High Tech Hospital Affiliated to Northwest University,Shaanxi Province,Xi'an 710065,China;不详)

机构地区：[1]西北大学附属西安高新医院检验科,西安710065 [2]西北大学附属西安高新医院内分泌科,西安710065

出　　处：《疑难病杂志》2022年第8期840-844,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：陕西省科技计划青年项目(2021JQ-920)。

摘　　要：目的探讨甲状腺癌组织中微加工处理器复合子亚单位8(DGCR8)和核糖核酸酶Ⅲ(DICER1)基因表达及临床意义。方法选取2015年1月-2017年1月西北大学附属西安高新医院内分泌科行手术治疗的甲状腺癌患者90例为研究对象。采用实时荧光定量PCR检测甲状腺癌组织和癌旁组织中DGCR8、DICER1基因表达水平。Pearson相关分析DGCR8和DICER1基因表达的相关性,分析DGCR8、DICER1基因表达与临床病理特征及预后的关系,多因素COX回归分析影响甲状腺癌患者复发的危险因素。结果与癌旁组织比较,甲状腺癌组织中DGCR8基因表达升高,而DICER1基因表达显著降低(t/P=26.458/<0.001、15.019/<0.001)。与AJCC分期Ⅰ~Ⅱ期甲状腺癌比较,AJCC分期Ⅲ~Ⅳ期患者病灶组织中DGCR8基因表达升高,DICER1基因表达降低(t/P=7.160/<0.001、17.441/<0.001)。Pearson相关分析显示,甲状腺癌组织DGCR8基因与DICER1基因的表达呈显著负相关(r=-0.662,P<0.001)。DGCR8基因高表达、DICER1基因低表达甲状腺癌患者3年复发率分别显著高于DGCR8基因低表达、DICER1基因高表达患者(χ^(2)/P=13.580/<0.001、27.889/<0.001)。DGCR8基因高表达、DICER1基因低表达患者无瘤中位生存时间分别短于DGCR8基因低表达、DICER1基因高表达患者(Z/P=3.425/<0.001、3.589/<0.001)。DGCR8基因高表达、DICER1基因低表达和AJCC分期Ⅲ~Ⅳ期是影响甲状腺癌术后复发的独立危险因素[OR(95%CI)=2.537(1.411~4.463)、1.659(1.119~2.576)、1.628(1.032~2.497)]。结论甲状腺癌组织中DGCR8基因表达升高、DICER1基因表达降低,两者与甲状腺癌患者AJCC分期相关,可辅助临床评估患者疾病预后。Objective To investigate the gene expression and clinical significance of microprocessor complex subunit 8(DGCR8)and ribonucleaseⅢ(DICER1)in thyroid cancer tissue.Methods A total of 90 patients with thyroid cancer who underwent surgery in the Department of Endocrinology,Xi'an High-tech Hospital Affiliated to Northwestern University from January 2015 to January 2017 were selected as the research objects.The gene expression levels of DGCR8 and DICER1 in thyroid cancer tissues and adjacent tissues were detected by real-time fluorescence quantitative PCR.Pearson correlation analysis was performed to analyze the correlation between the gene expressions of DGCR8 and DICER1,and to analyze the relationship between the gene expressions of DGCR8 and DICER1 with clinicopathological characteristics and prognosis.Multivariate COX regression analysis was used to analyze the risk factors of recurrence in patients with thyroid cancer.Results Compared with adjacent tissues,the gene expression of DGCR8 in thyroid cancer tissues was increased,while the gene expression of DICER1 was decreased(t/P=26.458/<0.001,15.019/<0.001).Compared with AJCC stage I-II thyroid cancer,the gene expression of DGCR8 in lesions of patients with AJCC stage III-IV was increased,and the gene expression of DICER1 was decreased(t/P=7.160/<0.001,17.441/<0.001).Pearson correlation analysis showed that the gene expressions of DGCR8 and DICER1 in thyroid cancer tissues were significantly negatively correlated(r=-0.662,P<0.001).The 3-year recurrence rate of thyroid cancer patients with high gene expression of DGCR8 and low gene expression of DICER1 was higher than that of patients with low gene expression of DGCR8 and high gene expression of DICER1(χ^(2)/P=13.580/<0.001,27.889/<0.001).The median tumor-free survival time of patients with high gene expression of DGCR8 and low gene expression of DICER1 was shorter than that of patients with low gene expression of DGCR8 and high gene expression of DICER1(Z/P=3.425/<0.001,3.589/<0.001).High gene expression of DGCR

关 键 词：甲状腺癌 微加工处理器复合子亚单位8 核糖核酸酶Ⅲ 基因表达 预后 

分 类 号：R736.1[医药卫生—肿瘤]