马铃薯淀粉的场流分离表征过程回收率研究  

Recovery of potato strach during separation and characterization process using field-flow fractionation

作　　者：张文惠[1] 宋天歌 孙瑜珊 申世刚[1] 窦海洋[2] ZHANG Wenhui;SONG Tiange;SUN Yushan;SHEN Shigang;DOU Haiyang(College of Chemistry and Environmental Science,Hebei University,Baoding 071002,China;School of Basic Medical Sciences,Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-autoimmune Disease of Hebei Province,Hebei University,Baoding 071000,China)

机构地区：[1]河北大学化学与环境科学学院,河北保定071002 [2]河北大学河北省炎性自身免疫性疾病发病机制及防治重点实验室,基础医学院,河北保定071000

出　　处：《河北大学学报（自然科学版）》2022年第4期376-383,共8页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目(H2020201295);大学生创新创业训练计划项目(S20221075029);大中学生科技创新能力培育专项(22E50032D)。

摘　　要：采用场流分离技术探究了马铃薯(Solanum tuberosum L.)淀粉分离表征过程中样品间以及样品与超滤膜的交联,评价了载液离子强度、样品进样量、交叉流流速和聚集时间对马铃薯淀粉回收率的影响,并对结果重复性进行了考察.结果表明:在载液(含5 mmol/L NaNO_(3),3 mmol/L NaN_(3))pH 7.00,样品进样量15μg,交叉流流速1.0 mL/min,聚集时间2.5 min的条件下,马铃薯淀粉的回收率为(97.30±0.02)%,回转半径相对标准偏差为1.18%,分子摩尔质量的相对标准偏差为5.67%.Potato starch was separated and characterized by field-flow fractionation.The interactions between the sample and the surface of ultrafiltration membrane in AF4 were investigated.The effects of ionic strength of carrier liquid,injection mass,cross-flow rate,and focusing time on the sample recovery were evaluated.The repeatability of the results was examined.The highest recovery of potato starch(97.30±0.02)% was obtained with a carrier liquid containing 5 mmol/L NaNO_(3) and 3 mmol/L NaN_(3),pH 7.00;injection mass,15μg;cross-flow rate,1.0 mL/min;focusing time,2.5 min.The method developed in this study showed good repeatability in terms of radius of gyration and molar mass with their relative standard deviations of 1.18% and 5.67%,respectively.

关 键 词：场流分离 马铃薯淀粉 回收率 

分 类 号：O657[理学—分析化学]