结直肠癌类器官的应用进展与挑战  

Modeling colorectal cancer with organoids:Clinical applications and challenges

作　　者：李精伟 陈浩[1] 沙卫红[1] 熊霞[2] 周健[2,3] LI Jingwei;CHEN Hao;SHA Weihong;XIONG Xia;ZHOU Jian(Department of Gastroenterology,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510080,China;National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]广东省人民医院消化内科,广东省医学科学院,广州510080 [2]畜禽养殖污染控制与资源化技术国家工程实验室,中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,长沙410125 [3]中国科学院大学,北京100049

出　　处：《科技导报》2022年第12期28-41,共14页Science & Technology Review

基　　金：国家自然科学基金项目(82171698,82170561,81741067,81300279);广东省自然科学基金杰出青年项目(2021B1515020003);广东省人民医院登峰计划项目(DFJH201803)。

摘　　要：结直肠癌(CRC)是全球发病率最高的消化道肿瘤之一,严重威胁人类的健康。近年来,类器官技术取得了令人欣喜的进展,已成为结直肠癌发生机制和临床转化研究的重要新工具。回顾了结直肠癌生态位信号通路的变化,总结了类器官技术在结直肠癌建模、肿瘤微环境研究、药物筛选、个体化治疗等方面的应用进展,讨论了当前类器官面临的挑战,并从类器官培养技术标准化和工程技术应用等角度展望了类器官的未来发展方向。The colorectal cancer is one of the most common digestive tract tumors globally,which seriously threatens the human health.In recent years,a gratifying progress has been made in the organoid culture technology,making the organoid technology an essential new tool for studying the colorectal cancer mechanisms and the clinical translation.This paper reviews the niche signaling pathways in the colorectal cancer,the application progress of the organoid technology in the colorectal cancer modeling,the tumor microenvironment research,the drug screening and the individualized therapy,as well as the current challenges of the organoid technology and the future development directions of the organoids from the perspectives of the organoid culture technology standardization and the engineering technology applications.

关 键 词：类器官 结直肠癌 干细胞生态位 肿瘤微环境 WNT信号 

分 类 号：R735.34[医药卫生—肿瘤]