Semen microbiota in normal and leukocytospermic males  

作　　者：Ye Yao Xin-Jian Qiu Dong-Sheng Wang Jie-Kun Luo Tao Tang Yun-Hui Li Chun-Hu Zhang Hao Liu Lu Zhou Lin-Lin Zhao 

机构地区：[1]Health Management Center,The Third Xiangya Hospital,Central South University,Changsha 410013,China [2]Institute of Integrated Traditional Chinese and Western Medicine,Xiangya Hospital,Central South University,Changsha 410005,China [3]Department of Nephrology,Integrated Hospital of Traditional Chinese and Western Medicine,Southern Medical University,Guangzhou 510220,China [4]Department of Traditional Chinese Medicine,Guangdong Second Provincial General Hospital,Guangzhou 510220,China

出　　处：《Asian Journal of Andrology》2022年第4期398-405,共8页亚洲男性学杂志（英文版）

基　　金：supported by Youth Foundation of National Natural Science Foundation of China(No.81703963 and No.81202807);the Natural Science Foundation of Hunan(No.2018JJ2667 and No.2019JJ40511);the Scientific Research Project of Chinese Traditional Medicine Administration Bureau in Hunan Province(No.201806).

摘　　要：Large numbers of microbes can be present in seminal fluid,and there are differences in the semen microbiota between normal and abnormal semen samples.To evaluate the semen microbiota in patients with leukocytospermia,87 seminal fluid samples,including 33 samples with a normal seminal leukocyte count and 54 samples with leukocytospermia,were obtained for a cross-sectional analysis.Twenty samples with a normal seminal leukocyte count had normal sperm parameters(Control group),and 13 samples with a normal seminal leukocyte count were from asthenozoospermia patients(Ast group).However,32 samples with leukocytospermia were from asthenozoospermia patients(LA group),and only 22 samples with leukocytospermia had normal sperm parameters(Leu group).The 16S ribosomal RNA(rRNA)gene sequencing method was used to sequence the microbiota in the seminal fluid,and multiple bioinformatics methods were utilized to analyze the data.Finally,the results showed that the worse sperm parameters were observed in the leukocytospermia-related groups.Semen microbiota analysis found that there was increased alpha diversity in the leukocytospermia-related groups.Firmicutes,Proteobacteria,Actinobacteria,and Bacteroidetes were the primary phyla in the seminal fluid.Two microbiota profiles,namely,Lactobacillus-enriched and Streptococcus-enriched groups,were identified in this study.The majority of the samples in the groups with a normal seminal leukocyte count could be categorized as Lactobacillus-enriched,whereas the majority of the leukocytospermia samples could be categorized as Streptococcus-enriched.Our study indicated that males with leukocytospermia have worse sperm parameters and a different semen microbiota composition compared to males with a normal seminal leukocyte count.

关 键 词：16S rRNA gene sequencing ASTHENOZOOSPERMIA LEUKOCYTOSPERMIA semen microbiota sperm motility 

分 类 号：R697[医药卫生—泌尿科学]