Wnt/β-catenin信号通路与大鼠腹膜纤维化-EMT相关研究  

Effects of Wnt/β-catenin signaling pathway on rat peritoneal fibrosis-EMT

作　　者：于晓猛 冯仁蕊[1] 王宏[1] 康利[1] 江茜 王郑辛夷 黄琳[2] 张川[1] 王蕾[1] YU Xiao-meng;FENG Ren-rui;WANG Hong;KANG Li;JIANG Qian;WANG Zheng-xinyi;HUANG Lin;ZHANG Chuan;WANG Lei(Tianjin Institute of Medical&Pharmaceutical Science,Tianjin 300020,China;Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan 528400,Guangdong,China)

机构地区：[1]天津市医药科学研究所,天津300020 [2]中山市中医院,广东中山528400

出　　处：《生物医学工程与临床》2022年第4期486-490,共5页Biomedical Engineering and Clinical Medicine

摘　　要：目的 探讨Wnt/β-catenin信号通路对大鼠腹膜间皮细胞上皮-间充质转化(EMT)进程的影响作用。方法 选择SPF级雄性Wistar大鼠20只,体质量180~200 g。随机分为对照组、模型组。采用5/6肾切除法+高糖腹膜透析液+脂多糖(LPS)法制作腹膜纤维化模型;大鼠于4周末次腹膜透析后,留取腹膜组织,采用苏木精-伊红(HE)染色观察腹膜组织形态学变化并测量腹膜组织厚度;采用实时定量聚合酶链式反应(PCR)、Western blot和免疫组织化学等方法检测腹膜组织中E-钙黏蛋白(E-cadherin)、α-平滑肌肌动蛋白(α-SMA)、Collagen-Ⅰ、β-catenin、Wnt-1和淋巴增强因子1(LEF1)的蛋白和mRNA表达。结果 与对照组相比,模型组大鼠腹膜组织明显增厚。模型组大鼠腹膜组织中E-cadherin、α-SMA、Collagen-Ⅰ、β-catenin、Wnt-1和LEF1蛋白与对照组比较明显升高(0.21±0.13 vs 1.33±0.41,0.27±0.20 vs 1.22±0.06,0.26±0.08 vs 1.08±0.13,0.21±0.11 vs 1.11±0.46,0.24±0.17 vs 1.99±0.36,0.37±0.15 vs 1.49±0.46。均P <0.01);mRNA表达水平明显升高(1.00±0.00 vs 3.07±0.85,1.00±0.00 vs 9.36±1.25,1.00±0.00 vs 13.5±0.31,1.00±0.00 vs 7.89±0.63,1.00±0.00 vs 18.2±1.79,1.00±0.00 vs 11.8±0.55。均P <0.01)。结论 Wnt/β-catenin信号通路显著影响EMT和腹膜纤维化的发展进程。Objective To investigate the effect of Wnt/β-catenin signaling pathway on epithelial-mesenchymal transition(EMT) process of rat peritoneal mesothelial cells. Methods Twenty male Wistar rats with body mass of 180-200 g were randomly divided into control group and model group. The peritoneal fibrosis model was established by 5/6 nephrectomy +high glucose peritoneal dialysate + lipopolysaccharide(LPS). The peritoneal tissue was collected from the rats after 4 weeks of peritoneal dialysis, and hematoxylin-eosin(HE) staining was used to observe morphological changes of peritoneal tissue and thickness of peritoneal tissue were measured. The protein and mRNA expressions of E-cadherin, α-SMA, Collagen-Ⅰ, β-catenin, Wnt-1 and LEF1 in peritoneal tissue were detected by real-time quantitative PCR, Western blot and immune-histo-chemistry. Results The rat peritoneal tissue in model group was significantly thicker than that in control group. The E-cad-herin, α-SMA, Collagen-Ⅰ, β-catenin, Wnt-1 and LEF1 proteins in the peritoneal tissue of rats in model group were significantly higher than those in control group(0.21 ± 0.13 vs 1.33 ± 0.41, 0.27 ± 0.20 vs 1.22 ± 0.06, 0.26 ± 0.08 vs 1.08 ± 0.13,0.21 ± 0.11 vs 1.11 ± 0.46, 0.24 ± 0.17 vs 1.99 ± 0.36, 0.37 ± 0.15 vs 1.49 ± 0.46. P < 0.01), and mRNA expression level in model group was significantly increased(1.00 ± 0.00 vs 3.07 ± 0.85, 1.00 ± 0.00 vs 9.36 ± 1.25, 1.00 ± 0.00 vs 13.5 ± 0.31,1.00 ± 0.00 vs 7.89 ± 0.63, 1.00 ± 0.00 vs 18.2 ± 1.79, 1.00 ± 0.00 vs 11.8 ± 0.55. P < 0.01). Conclusion It is demonstrated that Wnt/β-catenin signaling pathway significantly affects the development of EMT and peritoneal fibrosis.

关 键 词：WNT/Β-CATENIN信号通路 上皮-间充质转化(EMT) 腹膜纤维化 

分 类 号：R-33[医药卫生] R459.5