石榴皮、金银花、牡丹复配物体外抗氧化作用评价  

Study on in vitro Antioxidant Activity of Compound of Pomegranate Rind,Lonicera Japonica and Peony

作　　者：王荣[1] 秦兰 王娜娜[1] 张德柱 王晓茹 秦蓓[1,2] WANG Rong;QIN Lan;WANG Na-na;ZHANG De-zhu;WANG Xiao-ru;QIN Bei(College of Pharmaceutical,Xi'an Medical University,Shaanxi Xi'an 710021;School of Pharmacy,Chengdu Medical College,Sichuan Chengdu 610500;Shaanxi Panlong Pharmaceutical Group lmited by share Ltd.,Shanxi Xi'an 710025;Shaanxi Functional Food Engineering Center Co.,Ltd.,Shaanxi Xi'an 710065,China)

机构地区：[1]西安医学院药学院,陕西西安710021 [2]成都医学院药学院,四川成都610500 [3]陕西盘龙药业集团股份有限公司,陕西西安710025 [4]陕西功能食品工程中心有限公司,陕西西安710065

出　　处：《广州化工》2022年第15期57-59,64,共4页GuangZhou Chemical Industry

基　　金：国家自然科学基金项目(No:81903579,No:81903288);西安医学院校级科技创新团队(No:2021TD03);陕西省重点研发计划项目(No:2021ZDLSF03-05),西安市科技计划项目(No:2020KJRC0135);西安市未央区科技计划项目(No:201930);西安医学院校级重点药学学科(西医发[2019]96号);陕西省教育厅科研计划项目(No:21JK0901,No:21JK0894)。

摘　　要：评价复配物体外抗氧化活性。采用DPPH和ABTS自由基清除法评价复配物的自由基清除能力;建立AAPH氧化损伤大鼠红细胞模型,研究复配物对红细胞溶血及对丙二醛(MDA)和抗氧化酶活力的影响。复配物对DPPH和ABTS自由基的清除作用与浓度呈正比,IC_(50)分别为0.065 mg/mL和0.13 mg/mL。复配物对红细胞溶血具有保护作用,并显著降低AAPH损伤4 h时MDA含量,提高谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)酶活。本实验的复配产物具有良好的体外抗氧化活性。In vitro antioxidant activity of compound products was evaluated.DPPH free radical and ABTS free radical clearance method was established to evaluate the compound product ability for free radical clearance.The erythrocytes model of AAPH-damaged was established to study the effects of compound products on hemolysis rate,malondialdehyde(MDA)and antioxidant enzymes.The scavenging effect of the compound products on DPPH and ABTS free radicals increased with the increase of concentration.IC_(50) of the scavenge DPPH and ABTS free radicals were 0.065 mg/mL and 0.13 mg/mL.The protective effect of compound products hemolysis was concentration-dependent,and remarkably reduced MDA content at 4 h of AAPH damage,and significantly increased the activity of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)enzyme.The compound product of this experiment has certain antioxidant ability in vitro.

关 键 词：复配 抗氧化 DPPH ABTS AAPH 

分 类 号：R965.1[医药卫生—药理学]