基于UPLC-Q-Exactive Orbitrap-MS的半夏厚朴汤化学成分分析  

Analysis of Chemical Components of Ban-Xia-Hou-Po Decoction Based on UPLC-Q-Exactive Orbitrap-MS

作　　者：沈璐[1] 冯芳[1,2] SHEN Lu;FENG Fang(Department of Pharmaceutical Analysis,China Pharmaceutical University,Jiangsu Nanjing 210009;Key Laboratory of Drug Quality Control and Pharmacovigilance(Ministry of Education),Jiangsu Nanjing 210009,China)

机构地区：[1]中国药科大学药物分析教研室,江苏南京210009 [2]药物质量与安全预警教育部重点实验室,江苏南京210009

出　　处：《广州化工》2022年第15期105-109,共5页GuangZhou Chemical Industry

基　　金：国家自然科学基金资助项目(No:81872993)。

摘　　要：采用超高液相色谱-四极杆-静电场轨道阱联用技术(UPLC-Q-Exactive Orbitrap-MS)对半夏厚朴汤化学成分进行分析。采用XBridge BEH C_(18)色谱柱(100 mm×2.1 mm,2.5μm),以0.1%甲酸水溶液-甲醇为流动相进行梯度洗脱,流速为0.3 mL/min;质谱采用加热电喷雾离子源(HESI),正、负离子扫描模式。结果从半夏厚朴汤中共鉴定了90个化学成分,包括33个苯丙素类,19个氨基酸类,16个有机酸类,12个黄酮类等。本研究为半夏厚朴汤药效物质深入研究及质量控制奠定基础。The chemical components of Ban-Xia-Hou-Po Decoction was analyzed by UPLC-Q-Exactive Orbitrap-MS.The analysis was performed on XBridge BEH C_(18) column(100 mm×2.1 mm,2.5μm),with mobile phase of 0.1%formic acid water solution-methanol for gradient elution at a flow rate of 0.3 mL/min.Mass spectrometry was performed using heatingelectrospray ion source(HESI)under the positive and negative ion modes.As a result,90 chemical components were identified from Ban-Xia-Hou-Po Decoction,including 33 phenylpropanoids,19 amino acids,16 organic acids,12 flavonoids,etc.This research laid a foundation for further study and quality control of the studied decoction.

关 键 词：半夏厚朴汤 UPLC-Q-Exactive Orbitrap-MS 化学成分 

分 类 号：R917[医药卫生—药物分析学]