鸡毒支原体MG-HY株感染鸡气管的转录组学分析  

Transcriptomic Analysis on Responses of Chicken Trachea to Mycoplasma gallisepticum Strain MG-HY Infection

作　　者：吴春琳 钟乐苗 赵妍 李文迹 黄晓紫 吴异健[1,2,3] WU Chunlin;ZHONG Lemiao;ZHAO Yan;LI Wenji;HUANG Xiaozi;WU Yijian(College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province,Fuzhou350002,China;Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health,Fujian Agriculture And Forestry University,Fuzhou350002,China;ZooKo Biochec Technology Co.Ltd,Nanping354200,China)

机构地区：[1]福建农林大学动物科学学院,福州350002 [2]中西兽医结合与动物保健福建省高等学校重点实验室,福州350002 [3]福建省兽医中药与动物保健重点实验室,福州350002 [4]中科国创(南平)生物科技股份有限公司,南平354200

出　　处：《畜牧兽医学报》2022年第8期2652-2662,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：高校产学合作项目(2022N5001);福建省自然科学基金项目(2017J01597);福建农林大学科技创新专项基金项目(CXZX2018023)。

摘　　要：旨在进一步探究鸡毒支原体(MG)感染SPF雏鸡后气管基因组转录水平的变化,筛选出MG感染后参与气管黏膜炎性损伤的差异表达基因和调控通路。使用MG-HY株菌液按0.2 mL·羽经点眼滴鼻感染SPF雏鸡,感染后收集气管组织利用RNA-Seq技术进行测序分析。转录组分析结果显示,在MG感染期间RNA-seq共筛选出3 112个显著(P<0.01)差异表达基因(DEGs),其中,1 646个上调基因,1 466个下调基因。GO功能分析显示,差异基因主要涉及生物调节、刺激的反应、多细胞生物过程、细胞成分组织或生物发生等生物过程。KEGG-Pathway分析发现差异基因参与黏膜免疫信号传导通路,例如:细胞因子-细胞因子受体相互作用,细胞黏附分子(CAMs),细胞外基质(ECM)受体相互作用、紧密连接、PPAR信号通路和MAPK信号通路等,表明这些基因参与了MG诱导的雏鸡气管炎症反应和损伤。使用qRT-PCR验证与黏膜免疫相关的13个差异表达的基因,其结果与转录组分析一致。本研究为进一步阐明MG感染导致宿主气管黏膜上皮损伤和黏膜免疫机制提供了基础。To further explore the changes of transcription level of tracheal genome after Mycoplasma gallisepticum(MG)infection in SPF chickens,the differentially expressed genes and regulatory pathways involved in inflammatory injury of tracheal mucosa after MG infection were screened;The infection model was established by infusing SPF chickens with MG-HY strain at 0.2mL per feather through nasal and eye drops.Tracheal tissues were collected and then using RNA-Seq technology for sequencing analysis.The results of transcriptome analysis showed that a total of 3112significantly(P<0.01)differentially expressed genes(DEGs)were selected by RNA-seq during MG infection,including 1646up-regulated genes and 1466down-regulated genes.GO functional analysis found that differential genes mainly involve biological processes such as biological regulation,stimulus response,multicellular biological processes,cell component organization or biogenesis.KEGG-Pathway analysis showed that differential genes are involved in mucosal immune signaling pathways,such as:cytokine-cytokine receptor interactions,cell adhesion molecules(CAMs),extracellular matrix(ECM)receptor interactions,tight junctions,PPAR signaling pathways and MAPK signaling pathway,etc.,overall,these results showed that these genes may be involved in MG-induced tracheal inflammatory response and injury in chicks.Using qRT-PCR to verify 13differentially expressed genes related to mucosal immunity,the results are consistent with transcriptome analysis.This study provides a basis for further elucidating the host tracheal mucosal epithelial damage and mucosal immune mechanism caused by MG infection.

关 键 词：鸡毒支原体 RNA-SEQ 黏膜上皮 差异表达基因 免疫应答 

分 类 号：S852.62[农业科学—基础兽医学]