不动杆菌(Acinetobacter sp.)K7菌株转化卤代肉桂酸的特性研究  

The transformation efficiency of Acinetobacter sp. strain K7 to halogenated cinnamic acids

作　　者：马璐 王雅遥 郭雨辰 李敏[1] MA Lu;WANG Yayao;GUO Yuchen;LI Min(School of Biological Science and Engineering,North Minzu University,Yinchuan Ningxia 750021)

机构地区：[1]北方民族大学生物科学与工程学院,宁夏银川750021

出　　处：《环境污染与防治》2022年第8期1015-1019,共5页Environmental Pollution & Control

基　　金：中国科学院西部之光项目(No.XAB2020YW12)。

摘　　要：以前期分离的不动杆菌(Acinetobacter sp.)K7菌株为生物材料降解转化卤代肉桂酸,采用超高效液相色谱(UPLC)串联飞行时间高分辨质谱仪(MS)检测卤代肉桂酸的转化情况。结果表明,K7菌株对卤代肉桂酸类物质的转化作用具有显著的结构依赖特征,K7菌株对4-卤代肉桂酸具有高效转化能力,对2、3位卤代肉桂酸的转化能力明显降低。以8种卤代肉桂酸中转化率最高的4-氯肉桂酸为例,K7菌株在温度为10~40℃、pH为6~10、盐度(以NaCl质量分数计)≤0.6%、4-氯肉桂酸初始质量浓度为50~100mg/L时转化效率较高。K7菌株能将4-氯肉桂酸高效转化为4-氯苯甲酸,但不再发生进一步转化,作用靶点较为单一。Acinetobacter sp.strain K7,which was isolated earlier in the laboratory,was used as biomaterial to transfer halogenated cinnamic acids,and the transformation efficiency was detected by HPLC/MS.The results showed that the transformation of halogenated cinnamic acids by strain K7was structure-dependent.Strain K7showed high transformation ability to 4-halogenated cinnamic acids,but low transformational ability to 2/3-halogenated cinnamic acids.Among the 8halogenated cinnamic acids,4-chlorocinnamic acid presented the highest transformation efficiency.Taking 4-chlorocinnamic acid as an example,the effects of typical environmental factors on the transformation was studied.It was found that the strain K7could efficiently transfer 4-chlorocinnamic acid at 10-40 ℃,pH 6-10,salinity≤0.6%(measured by mass fraction of NaCl),and initial concentration of 4-chlorocinnamic acid 50-100mg/L.Strain K7could efficiently transfer 4-chlorocinnamic acid to 4-chlorobenzoic acid,but no further transformation occurred.The target of 4-chlorocinnamic acid transformation by strain K7was poor.

关 键 词：卤代肉桂酸 不动杆菌 转化 

分 类 号：X172[环境科学与工程—环境科学]