跑步对香烟提取物所致小鼠肺气肿模型的影响及机制研究  

Effect and Mechanism of Running on Mouse Emphysema Model Induced by Cigarette Extract

作　　者：李永荣 谢海彬 李红 孙杰 LI Yongrong;XIE Haibin;LI hong;SUN Jie(Affiliated Hospital of Gansu University of Traditional Chinese Medicine,Lanzhou,Gansu 730020,China;Luohu District Hospital of Traditional Chinese Medicine,Shenzhen,Guangdong 518001,China;The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou,Guangdong 510405,China)

机构地区：[1]甘肃中医药大学附属医院,甘肃兰州730020 [2]深圳市罗湖区中医院,广东深圳518001 [3]广州中医药大学第一附属医院,广东广州510405

出　　处：《康复学报》2022年第4期326-331,共6页Rehabilitation Medicine

基　　金：国家自然科学基金项目(81560743)。

摘　　要：目的:观察跑步对香烟提取物所致小鼠肺气肿模型影响,并基于信号传导及转录激活蛋白3(STAT3)探讨其作用机制。方法:选择40只雄性C57BL/6小鼠,按随机数字表法分为对照组、运动组、肺气肿组和肺气肿+运动组,每组10只。对照组和运动组小鼠在实验第0、11、22天分别通过腹腔注射0.3 mL磷酸盐缓冲液(PBS);肺气肿组和肺气肿+运动组小鼠在实验第0、11、22天分别通过腹腔注射0.3 mL香烟提取物,对照组和肺气肿组第23~29天在笼内自由活动;运动组和肺气肿+运动组第23~29天进行跑步机有氧训练(15 m/min,30 min/d),持续运动7 d。采用苏木精-伊红(HE)染色法观察小鼠肺组织病理改变,评估4组小鼠肺气肿程度;分析支气管肺泡灌洗液(BAL)和血清中炎症细胞及细胞因子表达水平,评估4组小鼠肺部及全身炎症情况;采用免疫印迹法分析肺组织中STAT3表达水平。结果:①肺气肿程度:与对照组比较,肺气肿组及肺气肿+运动组小鼠肺泡弦长均明显增大,差异具有统计学意义(P<0.05)。与肺气肿组比较,肺气肿+运动组小鼠肺泡弦长减小,差异具有统计学意义(P<0.05)。②肺部炎症:与对照组比较,肺气肿组及肺气肿+运动组小鼠BAL中白细胞、中性粒细胞、淋巴细胞含量及IL-1β、IL-6、IL-17表达水平明显升高,IL-10水平明显降低,差异具有统计学意义(P<0.05);与肺气肿组比较,肺气肿+运动组小鼠BAL中白细胞、中性粒细胞、淋巴细胞含量及IL-1β、IL-6、IL-17表达水平更低,IL-10水平更高,差异均有统计学意义(P<0.05)。③全身炎症:与对照组比较,肺气肿组小鼠血液中白细胞、中性粒细胞、淋巴细胞含量明显升高,肺气肿组及肺气肿+运动组小鼠血液中IL-1β、IL-17、TNF-α表达水平均明显升高,IL-10水平明显降低,差异均有统计学意义(P<0.05)。与肺气肿组比较,肺气肿+运动组小鼠血液中白细胞、中性粒细胞、淋巴细胞含量及IL-1βObjective:To observe the effect of running on mouse emphysema model induced by cigarette extract,and to explore its mechanism based on signal transducer and activator of transcription 3(STAT3).Methods:A total of 40 male C57BL/6 mice were randomly divided into control group,exercise group,emphysema group and emphysema+exercise group,with 10 mice in each group.The control group and the exercise group were intraperitoneally injected with 0.3 mL phosphate buffer saline(PBS)at the 0,11th and 22th day in the experiment;the emphysema group and the emphysema+exercise group were intraperitoneally injected with 0.3 mL cigarette extract at the 0,11th and 22th day in the experiment.The control group and the emphysema group were free to move in the cage from the 23rd to 29th day;the exercise group and the emphysema+exercise group received aerobic running training from the 23rd day to the 29th day(15 m/min,30 minutes a day).Hematoxylin eosin(HE)staining was used to observe the pathological changes of lung tissue and evaluate the severity of emphysema in the four groups;the expression levels of inflammatory cells and cytokines in bronchoalveolar lavage fluid(BAL)and serum were analyzed to evaluate the pulmonary and systemic inflammation of the four groups;Western blot method was used to analyze the expression of STAT3 in lung tissues.Results:(1)Severity of emphysema:compared with the control group,the alveolar chord length of mice in the emphysema group and the emphysema+exercise group significantly increased,the difference was statistically significant(P<0.05).Compared with the emphysema group,the alveolar chord length in the emphysema+exercise group significantly decreased,the difference was statistically significant(P<0.05).(2)Lung inflammation:compared with the control group,the contents of white blood cells,neutrophils and lymphocytes and the expression levels of IL-1β,IL-6,and IL-17 in BAL of the emphysema group and the emphysema+exercise group significantly increased,while the IL-10 level significantly decreased(P<0.05)

关 键 词：肺气肿 跑步 炎性细胞 炎性因子 信号传导及转录激活蛋白3 

分 类 号：R563.9[医药卫生—呼吸系统]