基于高通量测序技术研究不同品种桃果实的微生物多样性  

Microbial Diversity of Peach Fruits of Different Varieties Based on High-throughput Sequencing

作　　者：罗冬兰 瞿光凡 马超[1] 巴良杰 王瑞[1] 曹森[1] LUO Dong-lan;QU Guang-fan;MA Chao;BA Liang-jie;WANG Rui;CAO Sen(Guiyang College,Guizhou 550005,China)

机构地区：[1]贵阳学院,贵阳550005

出　　处：《包装工程》2022年第11期140-146,共7页Packaging Engineering

基　　金：贵州省科技计划(黔科合支撑[2020]1Y137号,黔科中引地[2020]4018号);贵阳市财政支持贵阳学院学科建设与研究生教育项目(2021-xk14)。

摘　　要：目的探究贵州不同品种桃果实附着的微生物菌群组成情况。方法以镇远红桃、镇远艳红桃和玉屏黄桃为试材,利用高通量测序技术分析贵州不同品种桃果实微生物的多样性。结果红桃的主要优势细菌属为泛菌属、鞘氨醇单孢菌属和假单胞菌属,优势真菌属为枝孢菌属、交链孢属和曲霉属;艳红桃的优势细菌属为拟杆菌属、布老特氏菌属和甲基杆菌属,优势真菌属为链核盘菌属、枝孢菌属和交链孢属;黄桃的优势细菌属为甲基杆菌属、鞘氨醇单孢菌属和棒状杆菌属,优势真菌属为枝孢菌属、链核盘菌属和交链孢属。此外,α多样性分析表明艳红桃的真菌多样性最高,而黄桃的真菌多样性次之;β多样性分析表明构成不同品种桃群落组成有明显差异,而黄桃与红桃的群落组成相似程度更高。结论研究初步明确了贵州不同品种桃果实的微生物多样性,为不同品种桃果实的绿色生产和贮藏保鲜提供理论基础。The work aims to explore the composition of microbial flora attached to the peach fruits of different varie-ties in Guizhou(a province in China).Taking Zhenyuan red peach,Yanhong peach and Yuping yellow peach as test ma-terials,high-throughput sequencing technology was used to analyze the microbial diversity of different peach varieties in Guizhou.The results showed that the main dominant bacterial genera of red peach were Pantoea,Sphingomonas and Pseudomonas,and the dominant fungal genera were Cladosporium,Alternaria and Aspergillus;The dominant bacterial genera of the brilliant red peach are Bacteroides,Blautia and Methylobacterium,the dominant fungal genera are Monili-nia,Cladosporium and Alternaria;The dominant bacterial genera of the yellow peach are Methylobacterium,Sphingo-monas and Corynebacterium,and the dominant fungi are Cladosporium,Monilinia and Alternaria.In addition,αdiversity analysis showed that the fungal diversity of red peach was the highest,and followed by yellow peach,βdiversity analysis showed that there were significant differences in the community composition of different peach varieties,while the community composition of yellow peach and red peach was more similar.This study preliminarily defined the microbial di-versity of different peach fruits in Guizhou and provided a theoretical basis for green production,storage and preservation of different peach fruits.

关 键 词：桃 高通量测序 微生物群落 多样性分析 

分 类 号：S609.3[农业科学—园艺学]