批式培养下热纤梭菌M3对纤维二糖降解特性的影响  

Effect of Ruminiclostridium thermocellum M3 degrading cellobiose characteristics under batch culture

作　　者：盛涛 孟庆斌 李学齐 孙彩玉 贾明珠 Sheng Tao;Meng Qingbin;Li Xueqi;Sun Caiyu;Jia Mingzhu(School of Environment&Chemical Engineering,Heilongjiang University of Science&Technology,Harbin 150022,China)

机构地区：[1]黑龙江科技大学环境与化工学院,哈尔滨150022

出　　处：《黑龙江科技大学学报》2022年第4期429-434,共6页Journal of Heilongjiang University of Science And Technology

基　　金：国家自然科学基金项目(51908200)。

摘　　要：为构建高效降解木质纤维素菌株提供有效可靠的靶点,以纤维二糖为底物,批式培养热纤梭菌R.thermocellum M3,研究不同底物质量浓度和温度下的纤维素酶活性变化和菌体生长情况,并结合转录组测序结果分析与纤维二糖降解相关的基因。研究结果表明:以10 g/L纤维二糖为底物时,R.thermocellum M3的β-葡萄糖苷酶活性最大值为0.314 IU/mL。随着纤维二糖质量浓度的升高,β-葡萄糖苷酶最大酶活逐渐降低;与60℃的培养条件相比,30℃时R.thermocellum M3菌体生长更好,但分泌的β-葡萄糖苷酶较低。转录组测序结果显示,R.thermocellum M3降解纤维二糖涉及BglA、BglB和BglX 3个重要基因,3个基因参与的主要代谢通路是糖酵解通路和淀粉及蔗糖代谢通路。The paper aims to provide an effective and reliable target for cultivating efficient lignocellulose-degrading strain.The study involves taking cellobiose as the substrate to breed Ruminiclostridium thermocellum M3,exploring the trends of cellulase activity and cell growth under different cellobiose concentrations and temperatures,and analyzing the genes related to degrade cellobiose combining the transcriptome sequencing.The results show that the peak activity ofβ-glucosidase is 0.314 IU/mL under 10 g/L cellobiose;along with the increase of cellobiose concentrationthe,the peak activity ofβ-glucosidase reduces gradually;compared with the culture conditions at 60℃,R.thermocellum M3 grows better under 30℃,while,the peak activity ofβ-glucosidase is lower.Transcriptome sequencing shows that the degradation of cellobiose conducted by R.thermocellum M3 are connected with three genes,BglA,BglB and BglX,and in which the main metabolic pathways involved are glycolysis pathway and starch and sucrose metabolism pathway.

关 键 词：热纤梭菌 纤维二糖 Β-葡萄糖苷酶 KEGG 

分 类 号：O636[理学—高分子化学] O936[理学—化学]