lncRNA LSINCT5通过miR-451调控肺癌细胞侵袭、迁移及顺铂敏感性的实验研究  

lncRNA LSINCT5 regulates invasion,migration and cisplatin sensitivity of lung cancer cells by miR-451

作　　者：熊玮[1] 刘慧敏 刘平[3] 熊印祥 刘巧 XIONG Wei;LIU Huimin;LIU Ping;XIONG Yinxiang;LIU Qiao(Department of Respiratory and Critical Care Medicine,Liyuan Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430077,China)

机构地区：[1]华中科技大学同济医学院附属梨园医院呼吸与危重症医学科,武汉430077 [2]华中科技大学同济医学院附属梨园医院儿科,武汉430077 [3]华中科技大学同济医学院附属梨园医院骨科,武汉430077 [4]华中科技大学同济医学院附属梨园医院检验科,武汉430077

出　　处：《中国免疫学杂志》2022年第11期1349-1354,共6页Chinese Journal of Immunology

基　　金：湖北省自然科学基金项目(2019CFB717)。

摘　　要：目的:探讨lncRNA LSINCT5调控miR-451对肺癌细胞侵袭、迁移及顺铂(DDP)敏感性的影响。方法:以肺癌A549细胞为研究对象,使用LSINCT5特异性siRNA、DDP(4μg/ml)、si-LSINCT+DDP+miR-451 inhibitor处理细胞,不经特殊处理的细胞为空白组,qRT-PCR检测LSINCT5和miR-451 mRNA表达;Transwell小室检测细胞侵袭和迁移能力;Western blot检测E-cadherin、Vimentin和N-cadherin表达;双荧光素酶报告基因检测试剂盒测定LSINCT5和miR-451的靶向关系。结果:在转染LSINCT5 siRNA的A549细胞中,LSINCT基因表达明显降低(P<0.05)。LSINCT5 siRNA及DDP均可明显抑制A549细胞侵袭、迁移能力,上调E-cadherin表达,下调Vimentin和N-cadherin表达(P<0.05),LSINCT5 siRNA及DDP联合使用对细胞侵袭、迁移及E-cadherin、Vimentin和N-cadherin表达影响明显强于单独使用LSINCT5 siRNA或DDP(P<0.05)。LSINCT5和miR-451存在靶向关系,LSINCT5可负向调控miR-451的表达。抑制LSINCT5可通过上调miR-451增强DDP对A549细胞侵袭、迁移的影响。结论:lncRNA LSINCT5可通过上调miR-451抑制肺癌细胞侵袭、迁移及DDP敏感性。Objective:To investigate the effect of lncRNA LSINCT5 on invasion,migration and cisplatin(DDP)sensitivity of lung cancer cells.Methods:Lung cancer A549 cells were used as research object.The cells were treated with LSINCT5 specific siRNA,DDP(4μg/ml),si-LSINCT5+DDP+miR-451 inhibitor,and the cells without special treatment were used as blank group.The mRNA expression of LSINCT5 and miR-451 were detected by qRT-PCR,and the cell invasion and migration ability were detected by Transwell cell chamber.Expressions of E-cadherin,Vimentin and N-cadherin were detected by Western blot.The targeting relationship between LSINCT5 and miR-451 were detected by double luciferase reporter gene detection kit.Results:The expression of LSINCT5 gene was significantly decreased in A549 cells transfected with LSINCT5 siRNA(P<0.05).LSINCT5 siRNA and DDP significantly inhibited the invasion and migration of A549 cells,up-regulated the expression of E-cadherin,down-regulated the expression of Vimentin and N-cadherin(P<0.05).The combined use of LSINCT5 siRNA and DDP significantly affected cell invasion and migration and expressions of E-cadherin,vimentin and N-cadherin(P<0.05).There was a targeting relationship between LSINCT5 and miR-451,LSINCT5 could negatively regulate the expression of miR-451.Inhibition of LSINCT5 could enhance the effect of DDP on invasion and migration of A549 cells by up-regulating miR-451.Conclusion:lncRNA LSINCT5 can inhibit the invasion,migration of lung cancer cells and DDP sensitivity by up-regulating miR-451.

关 键 词：肺癌 lncRNA LSINCT5 miR-451 侵袭 迁移 顺铂 

分 类 号：R734.2[医药卫生—肿瘤]