金荞麦HPLC条件优化及表儿茶素鉴定研究  

Fagopyrum Buckwheat HPLC Conditions Optimization and Epicatechin Appraisal Research

作　　者：吴海涛[1] 陈玥 杨微 吴灵玉 Wu Haitao;Chen Yue;Yang Wei;Wu Lingyu(College of Science,Heilongjiang Bayi Agricultural University,Daqing 163319;College of Life Science and Technology,Heilongjiang Bayi Agricultural University;State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention;Key Laboratory of Veterinary Pharmaceutical Development,Ministry of Agriculture/China Key Laboratory of New Animal Drug Project of Giansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences/Chinese Academy of Agricultural Science)

机构地区：[1]黑龙江八一农垦大学理学院,大庆163319 [2]黑龙江八一农垦大学生命科学技术学院 [3]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室 [4]中国农业科学院兰州畜牧与兽药研究所/农村农业部兽用药物创制重点实验室/甘肃省新兽药工程重点实验室

出　　处：《黑龙江八一农垦大学学报》2022年第4期80-86,133,共8页journal of heilongjiang bayi agricultural university

摘　　要：采用薄层层析(TLC)、高效液相色谱(HPLC)技术,对金荞麦药材醇提物中的黄烷醇类成分进行分析和鉴别,并对药典方法中的HPLC条件进行优化(如流动相种类、洗脱方式、检测波长)。色谱条件为:采用Symmetry C18(150 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-甲酸水溶液(pH=3.0)等度洗脱,7.5∶92.5的比例,1.0 mL·min^(-1)的流速,280 nm的检测波长,35℃的柱温。结果表明,金荞麦提取物和表儿茶素标准品溶液在相同的保留时间下有相同的峰出现。方法准确可靠,重现性好,可用于金荞麦片的质量控制检测。The flavanols composition of alcohol extract of Fagopyrum buckwheat was analyzed and identified by Thin-Layer Chromatography and High-Performance Liquid Chromatography and were optimized the high performance liquid(HPLC)method in Ch P,such as the type of mobile phase,the type of elution and the detection wavelength.The detection conditions was Symmetry C18(150 mm×4.6 mm,5 μm)column with the mobile phase of acetonitrile and methane acid solution(pH=3.0)in a gradient elution,the radio of 7.5∶92.5,the flow rate was 1.0 mL·min^(-1),and the wavelength to detect was 280 nm,the temperature of column was 35 ℃.There was the same peak appeared under the same retention time about the Fagopyrum buckwheat extracts and epicatechin standard solution.This method was accurate,reliable,reproducible and could be used for quality control of Fagopyrum buckwheat.

关 键 词：金荞麦 薄层层析 高效液相色谱 表儿茶素 

分 类 号：R284.1[医药卫生—中药学]