党参青霉病病原菌鉴定及生物学特性  

Pathogen identification and biological characteristics on blue mold of Codonopsis pilosula

作　　者：吕丙钰 杨董云 薛华丽[1] 杨志敏 LYU Bingyu;YANG Dongyun;XUE Huali;YANG Zhimin(College of Science,Gansu Agricultural University,Lanzhou 730070,China;Lanzhou Institute for Food and Drug Control,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学理学院,甘肃兰州730070 [2]兰州市食品药品检验检测研究院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2022年第3期66-74,83,共10页Journal of Gansu Agricultural University

基　　金：国家自然科学基金项目(31560475,32060566);“十三五”国家重点研发计划重点专项(2016YFD0400902)。

摘　　要：【目的】明确党参采后青霉病病原,为党参青霉病的防控提供理论支持。【方法】通过形态学和分子生物学的方法对新鲜党参采后青霉病病原进行鉴定,并分析其生物学特性。【结果】本试验中将党参青霉病病原鉴定为扩展青霉(Penicillium expansum)。该病原菌菌丝生长的最佳条件为25℃、pH 7和24 h光照,碳源为葡萄糖,氮源为蛋白胨;产孢的最适条件为25℃,pH 6及24 h光照,碳源为β-环糊精,氮源为酵母粉;65℃处理10 min可致死。【结论】新鲜党参青霉病病原为扩展青霉,且可代谢产生棒曲霉素。【Objective】To identify the pathogen causing bule mold of Codonopsis pilosula and provide theoretical support for the prevention and control of the postharvest disease of Codonopsis pilosula.【Method】The main pathogenic fungus causing bule mold of Codonopsis pilosula was identified using morphological and molecular biology methods,and its biological characteristics were analyzed.【Result】That the main pathogen of blue mold for Codonopsis pilosula was Penicillium expansum.The optimum condition for mycelial growth was that the temperature was 25℃,the pH was 7,the optimum illumination condition was 24 h,the carbon source was glucose,and the nitrogen source was peptone.The optimum condition for sporulation was that the temperature was 25℃,the pH was 6,the light condition was 24 h light,the carbon source wasβ-cyclodextrin,and the nitrogen source was yeast powder.Penicillium expansum would be lethal at 65℃for 10min.【Conclusion】The main pathogenic fungus of blue mold of fresh Codonopsis pilosula was Penicilliosis expansum,which can metabolize patulin.

关 键 词：党参 青霉病 扩展青霉 病原菌鉴定 生物学特性 棒曲霉素 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]