High-fidelity carbon dots polarity probes:revealing the heterogeneity of lipids in oncology  

作　　者：Jingyu Hu Yuanqiang Sun Xin Geng Junli Wang Yifei Guo Lingbo Qu Ke Zhang Zhaohui Li 

机构地区：[1]Colllege of Chemistry,Institute of Analytical Chemistry for Life Science,Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic,Zhengzhou University,450001 Zhengzhou,China [2]Institute of Chemical Biology and Clinical Application at the First Affiliate Hospital,Zhengzhou University,450001 Zhengzhou,China [3]Department of Chemistry and Chemical Biology,Northeastern University,Boston,MA 02115,USA

出　　处：《Light(Science & Applications)》2022年第7期1555-1564,共10页光（科学与应用)（英文版）

基　　金：support from the National Natural Science Foundation of China(No.219741256);Program for Innovative Research Team(in Science and Technology)in the University of Henan Province(No.22TRTSTHN002);111 Project of Henan Province(No.CXJD2021001);Zhonguan Scholars Program(No.202101510005).

摘　　要：Polarity is an integral microenvironment parameter in biological systems closely associated with a multitude of cellular processes.Abnormal polarity variations accompany the initiation and development of pathophysiological processes.Thus,monitoring the abnormal polarity is of scientific and practical importance.Current state-of-the-art monitoring techniques are primarily based on fluorescence imaging which relies on a single emission intensity and may cause inaccurate detection due to heterogeneous accumulation of the probes.Herein,we report carbon dots(CDs)with ultra-sensitive responses to polarity.The CDs exhibit two linear relationships:one between fluorescence intensity and polarity and the other between polarity and the maximum emission wavelength.The emission spectrum is an intrinsic property of the probes,independent of the excitation intensity or probe concentration.These features enable two-color imaging/quantitation of polarity changes in lipid droplets(LDs)and in the cytoplasm via in situ emission spectroscopy.The probes reveal the polarity heterogeneity in LDs which can be applied to make a distinction between cancer and normal cells,and reveal the polarity homogeneity in cytoplasm.

关 键 词：polarity probes REVEALING 

分 类 号：R730.2[医药卫生—肿瘤]