Highly selective NIR fluorescent probe for acetylcholinesterase and its application in pesticide residues detection  

作　　者：Shengui He Shufang Zhang Xin Zhao Xinyue Zhu Lisen Chen Jingnan Cui 

机构地区：[1]State Key Laboratory of Fine Chemicals Dalian University of Technology,Dalian 116024,China

出　　处：《Chinese Chemical Letters》2022年第9期4233-4237,共5页中国化学快报（英文版）

基　　金：supported financially by National Key R&D Program of China (No. 2018YFC1603001)

摘　　要：A series of near-infrared(NIR)fluorescent substrates(NDRO-1∼8)derived from fluorophore NDRH with different volumes of ester bond as the recognition group were designed and synthesized for the detection of acetylcholinesterase(AChE),among which NDRO-1 with the smallest acetate group displayed the highest activity toward AChE.The detection limit of NDRO-1 for sensing AChE was 0.32μg/mL,and K_(m )was 6.40μmol/L,indicating ultra-sensitivity and good affinity of NDRO-1 toward AChE.NDRO-1 was used to detect the inhibitory of four kinds of pesticides including methamidophos,dichlorvos,and the detection limit was lower than 50μg/L,which was further used in pesticide residues detection.

关 键 词：NIR fluorescent probe ACETYLCHOLINESTERASE Enzyme-activated Pesticide residue detection 

分 类 号：O657.3[理学—分析化学] TS255.7[理学—化学]