远东拟沙丁鱼黄嘌呤氧化酶抑制肽的制备及其降尿酸活性的研究  

Preparation of the xanthine oxidase inhibitory peptide from Sardinops sagax and its activity on reducing uric acid

作　　者：詹苏泓 吉宏武[1,2,3,4,5] 张迪 韦柳益[1] 彭烁 陈铭 宋文奎 瞿瑜杉 刘书成 ZHAN Suhong;JI Hongwu;ZHANG Di;WEI Liuyi;PENG Shuo;CHEN Ming;SONG Wenkui;QU Yushan;LIU Shucheng(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Zhanjiang 524088,China;Guangdong Province Engineering Laboratory for Marine Biological Products,Zhanjiang 524088,China;Guangdong Provincial Engineering Technology Research Center of Marine Food,Zhanjiang 524088,China;Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东省水产品加工与安全重点实验室,广东湛江524088 [3]广东省海洋生物制品工程实验室,广东湛江524088 [4]广东省海洋食品工程技术研究中心广东湛江,524088 [5]广东省高等学校水产深加工重点实验室,广东湛江524088 [6]大连理工大学海鲜深加工协同创新中心,辽宁大连116034

出　　处：《食品与发酵工业》2022年第16期79-86,共8页Food and Fermentation Industries

基　　金：国家重点基础研究发展计划“蓝色粮仓专项”(2019YFD0902004)。

摘　　要：为了从远东拟沙丁鱼肉酶解物中制备黄嘌呤氧化酶(xanthine oxidase,XO)抑制肽并研究其降尿酸活性,该研究以远东拟沙丁鱼肉为原料,XO抑制率为考察指标,采用碱性蛋白酶水解远东拟沙丁鱼肉,产生的酶解物通过超滤与凝胶色谱、反相高效液相色谱(reversed phase high performance liquid chromatography,RP-HPLC)分离纯化,从中分离出2个XO抑制肽,它们的氨基酸序列采用超高效液相四级杆飞行时间串联质谱(ultra-high performance liquid chromatography quadrupole time of flight tandem mass spectrometry,UPLC-MS/MS)鉴定为苯丙氨酸-亮氨酸-精氨酸(Phe-Leu-Arg,FLR)与精氨酸-脯氨酸-赖氨酸(Arg-Pro-Lys,RPK)。采用腺苷及XO联合诱导人肾皮质近曲小管上皮细胞(HK-2)构建高尿酸细胞模型,对2个肽的降尿酸功效进行评价,结果显示FLR、RPK具有显著降尿酸作用。In order to prepare xanthine oxidase(XO)inhibitory peptides from the hydrolysates of Sardinops sagax,and study their activity of reducing uric acid,the XO inhibition rate was used as the index,S.sagax meat was hydrolyzed by alkaline protease.The hy-drolysates obtained were purified by ultrafiltration,gel chromatography and reverse phase high performance liquid chromatography(RP-HPLC),two XO inhibitory peptides were purified.Their amino acid sequences were identified as Phe-Leu-Arg(FLR)and Arg-Pro-Lys(RPK)by ultra-high performance liquid chromatography quadrupole time of flight tandem mass spectrometry(UPLC-MS/MS).A hyperu-ricemic cell model was established by combined inducing of HK-2 with adenosine and XO.Lowering uric acid effects of these two peptides were evaluated.The results showed that FLR and RPK both had significant effects of lowering uric acid.

关 键 词：XO抑制肽 氨基酸序列 HK-2高尿酸模型 降尿酸 远东拟沙丁鱼 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]